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Rapid Identification of Radical Scavenging Phenolic Compounds from Black Bamboo Leaves using High-Performance Liquid Chromatography Coupled to an Online ABTS(+)-Based Assay

Title
Rapid Identification of Radical Scavenging Phenolic Compounds from Black Bamboo Leaves using High-Performance Liquid Chromatography Coupled to an Online ABTS(+)-Based Assay
Author
김철영
Keywords
antioxidant; flavonoids; online antioxidants screening system; Phyllostachys nigra
Issue Date
2009-10
Publisher
KOREAN SOC AGRICULTURAL CHEMISTRY & BIOTECHNOLOGY
Citation
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v. 52, No. 6, Page. 613-619
Abstract
The methanol extract of black bamboo (Phyllostachys nigra Munro) leaves showed significant radical scavenging activity in an ABTS(+)-based assay. The high-performance liquid chromatography (HPLC) coupled to an online ABTS(+)-based assay system was used to identify and quantify the antioxidant principles from bamboo leaves. Antioxidant compounds were isolated from the ethyl acetate soluble fraction through the activity profiles based on the online A-BTS+ assay, and identified as isoorientin (1), orientin (2), vitexin (3), luteolin 6-C-(6-O-trans-caffeoylglucoside) (4), vittariflavone (5), and tricin (6) using NMR and H-PLGESUMS data. Antioxidant activity levels of the isolated isoorientin (1), orientin (2), and luteolin 6-C-(6-O-trans-caffeoylglucoside) (4) were 33.10, 30.96, and 17.11 mM for a 50% reduction of the ABTS(+) radical, respectively. This HPLC online ABTS'-based assay system was a rapid and efficient method for measuring antioxidants' activity-guided purification from natural resources.
URI
https://link.springer.com/article/10.3839/jksabc.2009.102http://repository.hanyang.ac.kr/handle/20.500.11754/76445
ISSN
1738-2203
DOI
10.3839/jksabc.2009.102
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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