Rapid Identification of Radical Scavenging Phenolic Compounds from Black Bamboo Leaves using High-Performance Liquid Chromatography Coupled to an Online ABTS(+)-Based Assay
- Title
- Rapid Identification of Radical Scavenging Phenolic Compounds from Black Bamboo Leaves using High-Performance Liquid Chromatography Coupled to an Online ABTS(+)-Based Assay
- Author
- 김철영
- Keywords
- antioxidant; flavonoids; online antioxidants screening system; Phyllostachys nigra
- Issue Date
- 2009-10
- Publisher
- KOREAN SOC AGRICULTURAL CHEMISTRY & BIOTECHNOLOGY
- Citation
- JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v. 52, No. 6, Page. 613-619
- Abstract
- The methanol extract of black bamboo (Phyllostachys nigra Munro) leaves showed significant radical scavenging activity in an ABTS(+)-based assay. The high-performance liquid chromatography (HPLC) coupled to an online ABTS(+)-based assay system was used to identify and quantify the antioxidant principles from bamboo leaves. Antioxidant compounds were isolated from the ethyl acetate soluble fraction through the activity profiles based on the online A-BTS+ assay, and identified as isoorientin (1), orientin (2), vitexin (3), luteolin 6-C-(6-O-trans-caffeoylglucoside) (4), vittariflavone (5), and tricin (6) using NMR and H-PLGESUMS data. Antioxidant activity levels of the isolated isoorientin (1), orientin (2), and luteolin 6-C-(6-O-trans-caffeoylglucoside) (4) were 33.10, 30.96, and 17.11 mM for a 50% reduction of the ABTS(+) radical, respectively. This HPLC online ABTS'-based assay system was a rapid and efficient method for measuring antioxidants' activity-guided purification from natural resources.
- URI
- https://link.springer.com/article/10.3839/jksabc.2009.102https://repository.hanyang.ac.kr/handle/20.500.11754/76445
- ISSN
- 1738-2203
- DOI
- 10.3839/jksabc.2009.102
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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