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dc.contributor.author채영규-
dc.date.accessioned2018-10-10T05:59:11Z-
dc.date.available2018-10-10T05:59:11Z-
dc.date.issued2016-08-
dc.identifier.citationBMC GENOMICS, v. 17, Page. 1-15en_US
dc.identifier.issn1471-2164-
dc.identifier.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-3004-8-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/76402-
dc.description.abstractBackground: Host-specific environmental factors induce changes in Bacillus anthracis gene transcription during infection. A global transcription regulator, CodY, plays a pivotal role in regulating central metabolism, biosynthesis, and virulence in B. anthracis. In this study, we utilized RNA-sequencing to assess changes in the transcriptional patterns of CodY-regulated B. anthracis genes in response to three conditions of environmental starvation: iron, CO2, or glucose deprivation. In addition, we performed chromatin immunoprecipitation on newly identified CodY-mediated genes. Results: Environmental deprivation induced transcriptional changes in CodY-regulated genes in both wild-type and codY strains, and both CodY-specific and environment-specific patterns were observed. In the iron-depleted condition, overexpression of iron homeostasis genes was observed independent of codY deletion; however, transcription of siderophore and amino acid biosynthesis genes was CodY dependent. Although CodY has a significant regulatory role in central metabolism and the carbon overflow pathway, metabolism-associated genes exhibited CodY-independent expression patterns under glucose starvation. Genes that were differentially expressed in response to CO2 availability showed CodY-dependent regulation, though their maximal expression did require a supply of CO2/bicarbonate. Conclusions: We speculate that CodY regulates the expression of environmental-responsive genes in a hierarchical manner and is likely associated with other transcription regulators that are specific for a particular environmental change.en_US
dc.description.sponsorshipThis research was supported by Global Ph. D. Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2012-003722) and NRF Grant funded by Korean Government (MSIP 2011-0030049). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.language.isoenen_US
dc.publisherBIOMED CENTRAL LTDen_US
dc.subjectBacillus anthracisen_US
dc.subjectCodYen_US
dc.subjectEnvironmental signalsen_US
dc.subjectStarvationen_US
dc.subjectRNA-sequencingen_US
dc.subjectChromatin immunoprecipitationen_US
dc.titleChanges in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditionsen_US
dc.typeArticleen_US
dc.relation.volume17-
dc.identifier.doi10.1186/s12864-016-3004-8-
dc.relation.page1-15-
dc.relation.journalBMC GENOMICS-
dc.contributor.googleauthorKim, Se Kye-
dc.contributor.googleauthorJung, Kyoung Hwa-
dc.contributor.googleauthorChai, Young Gyu-
dc.relation.code2016006629-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidygchai-


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