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Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions

Title
Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions
Author
채영규
Keywords
Bacillus anthracis; CodY; Environmental signals; Starvation; RNA-sequencing; Chromatin immunoprecipitation
Issue Date
2016-08
Publisher
BIOMED CENTRAL LTD
Citation
BMC GENOMICS, v. 17, Page. 1-15
Abstract
Background: Host-specific environmental factors induce changes in Bacillus anthracis gene transcription during infection. A global transcription regulator, CodY, plays a pivotal role in regulating central metabolism, biosynthesis, and virulence in B. anthracis. In this study, we utilized RNA-sequencing to assess changes in the transcriptional patterns of CodY-regulated B. anthracis genes in response to three conditions of environmental starvation: iron, CO2, or glucose deprivation. In addition, we performed chromatin immunoprecipitation on newly identified CodY-mediated genes. Results: Environmental deprivation induced transcriptional changes in CodY-regulated genes in both wild-type and codY strains, and both CodY-specific and environment-specific patterns were observed. In the iron-depleted condition, overexpression of iron homeostasis genes was observed independent of codY deletion; however, transcription of siderophore and amino acid biosynthesis genes was CodY dependent. Although CodY has a significant regulatory role in central metabolism and the carbon overflow pathway, metabolism-associated genes exhibited CodY-independent expression patterns under glucose starvation. Genes that were differentially expressed in response to CO2 availability showed CodY-dependent regulation, though their maximal expression did require a supply of CO2/bicarbonate. Conclusions: We speculate that CodY regulates the expression of environmental-responsive genes in a hierarchical manner and is likely associated with other transcription regulators that are specific for a particular environmental change.
URI
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-3004-8http://repository.hanyang.ac.kr/handle/20.500.11754/76402
ISSN
1471-2164
DOI
10.1186/s12864-016-3004-8
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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