Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 유혜현 | - |
dc.date.accessioned | 2018-10-02T04:50:05Z | - |
dc.date.available | 2018-10-02T04:50:05Z | - |
dc.date.issued | 2009-09 | - |
dc.identifier.citation | XENOBIOTICA, v. 39, No. 9, Page. 680-686 | en_US |
dc.identifier.issn | 0049-8254 | - |
dc.identifier.uri | https://www.tandfonline.com/doi/abs/10.1080/00498250902998699 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/76309 | - |
dc.description.abstract | N,N-dimethylamphetamine (DMA) is a methamphetamine analogue known to be a weaker central nervous system stimulant than methamphetamine. Although a major metabolite of DMA is known to be DMA N-oxide (DMANO), which may be catalysed by flavin-containing monooxygenase (FMO), the specific enzyme(s) involved in this biotransformation has not been identified. In this study, the specific enzyme(s) involved with DMA N-oxidation was characterized by several assays. When DMA was incubated with different human recombinant drug-metabolizing enzymes, including FMOs and cytochrome P450s (CYPs), the formation of DMANO by FMO1 was the most predominant. The Michaelis-Menten kinetic constants for DMA N-oxidation by FMO1 were: K(m) of 44.5 mu M, V(max) of 7.59 nmol min(-1) mg(-1) protein, and intrinsic clearance of 171 mu l min(-1) mg(-1) protein, which was about twelve-fold higher than that by FMO3. Imipramine, an FMO1-specific inhibitor, selectively inhibited DMA N oxidation. The resulting data showed that DMA N oxidation is mainly mediated by FMO1. | en_US |
dc.description.sponsorship | This work was supported in part by the grant (2V01440) from Korea Institute of Science and Technology and in part by the grant (M10640010000-06N4001-00100) from National R&D program of Ministry of Education, Science and Technology (MEST) and Korea Science and Engineering Foundation (KOSEF). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | TAYLOR & FRANCIS LTD | en_US |
dc.subject | N,N-dimethylamphetamine (DMA) | en_US |
dc.subject | flavin-containing monooxygenase | en_US |
dc.subject | N-oxidation | en_US |
dc.subject | N,N-dimethylamphetamine N-oxide (DMANO) | en_US |
dc.title | Flavin-containing monooxygenase 1-catalysed N,N-dimethylamphetamine N-oxidation | en_US |
dc.type | Article | en_US |
dc.relation.volume | 39 | - |
dc.identifier.doi | 10.1080/00498250902998699 | - |
dc.relation.page | 680-686 | - |
dc.relation.journal | XENOBIOTICA | - |
dc.contributor.googleauthor | Lee, S. K | - |
dc.contributor.googleauthor | Kang, M. J | - |
dc.contributor.googleauthor | Jin, C | - |
dc.contributor.googleauthor | In, M. K | - |
dc.contributor.googleauthor | Kim, D. -H | - |
dc.contributor.googleauthor | Yoo, H. H | - |
dc.relation.code | 2009209901 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | yoohh | - |
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