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dc.contributor.author최한곤-
dc.date.accessioned2018-09-21T01:33:07Z-
dc.date.available2018-09-21T01:33:07Z-
dc.date.issued2009-06-
dc.identifier.citationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v. 50, No. 2, Page. 267-270en_US
dc.identifier.issn0731-7085-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S073170850900260X-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/76144-
dc.description.abstractSibutramine, a monoamine reuptake inhibitor, is used as a racemate, for the treatment of obesity. it is converted in vivo mainly to two desmethyl active metabolites, mono-desmethylsibutramine (MDS) and di-desmethylsibutramine (DDS). In the present study, we introduced a rapid and simple chromatographic method for separating the R(+)- and S(-)-isomers of sibutramine, MDS, and DDS, respectively. The stereoisomers of the three compounds were extracted from rat plasma using diethyl ether and n-hexane under alkaline conditions. After evaporating the organic layer. the residue was reconstituted in the mobile phase (10 mM ammonium acetate buffer adjusted to pH 4.03 with acetic acid: acetonitrile, 94:6, v/v). The enantiomers in the extract were separated on a Chiral-AGP stationary-phase column and were quantified in a tandem mass spectrometry. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This method Was used to measure the concentrations of the enantiomers of sibutramine, MDS, and DDS in plasma after a single oral dose of 10 mg/kg racemic sibutramine in rats. (C) 2009 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_US
dc.subjectSibutramineen_US
dc.subjectMono-desmethylsibutramineen_US
dc.subjectDi-desmethylsibutramineen_US
dc.subjectChiral separationen_US
dc.subjectLC/MS/MSen_US
dc.titleAnalysis of enantiomers of sibutramine and its metabolites in rat plasma by liquid chromatography-mass spectrometry using a chiral stationary-phase columnen_US
dc.typeArticleen_US
dc.relation.no2-
dc.relation.volume50-
dc.identifier.doi10.1016/j.jpba.2009.04.024-
dc.relation.page267-270-
dc.relation.journalJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS-
dc.contributor.googleauthorBae, Kyoungjin-
dc.contributor.googleauthorNoh, Kyeumhan-
dc.contributor.googleauthorJang, Kiyoung-
dc.contributor.googleauthorKim, Sohee-
dc.contributor.googleauthorYong, Chul Soon-
dc.contributor.googleauthorChoi, Han-Gon-
dc.contributor.googleauthorKang, Jong Seong-
dc.contributor.googleauthorChen, Jianbo-
dc.contributor.googleauthorMa, Eunsook-
dc.contributor.googleauthorLee, Manhyung-
dc.contributor.googleauthorShin, Beom Soo-
dc.contributor.googleauthorKwon, Kwang-il-
dc.contributor.googleauthorKang, Wonku-
dc.relation.code2009205623-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidhangon-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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