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Probe-Based Fluorescence Melting Curve Analysis for Differentiating Larimichthys polyactis and Larimichthys crocea

Title
Probe-Based Fluorescence Melting Curve Analysis for Differentiating Larimichthys polyactis and Larimichthys crocea
Author
황승용
Keywords
Larimichthys crocea; Larimichthys polyactis; Croaker; Species identification; Fluorescence melting curve analysis; Locked nucleic acid
Issue Date
2016-07
Publisher
SPRINGER
Citation
FOOD ANALYTICAL METHODS (2016.7), v. 9, NO. 7, Page. 2036-2041
Abstract
Larimichthys polyactis (redlip yellow croaker) and Larimichthys crocea (large yellow croaker) are commercially important fish species in East Asia with high differences in their market values. In Korea, consumers prefer L. polyactis to L. crocea, although it is difficult to distinguish them based on their morphological traits. The objective of this study was to develop an assay for differentiating L. polyactis and L. crocea using fluorescence melting curve analysis (FMCA) with a single locked nucleic acid (LNA) probe. Species-specific regions of the mitochondrial 16S rDNA were selected as LNA probes. The target sequences of L. polyactis and L. crocea had a 2-bp difference, and a single LNA probe was identified using melting temperature (Tm) shift. LNA probe was 100 % complementary to the target sequence of ten L. polyactis samples, giving a significantly higher Tm value (66 A degrees C) than that of five L. crocea samples (42 A degrees C). Overall, the developed LNA-based FMCA system had high efficiency, multiplexity, and simplicity, and could be effectively used for differentiating L. polyactis and L. crocea, and as a food analyzing method based on DNA sequence.
URI
https://link.springer.com/article/10.1007%2Fs12161-015-0381-6http://repository.hanyang.ac.kr/handle/20.500.11754/74533
ISSN
1936-9751; 1936-976X
DOI
10.1007/s12161-015-0381-6
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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