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Genotyping of velvet antlers for identification of country of origin using mitochondrial DNA and fluorescence melting curve analysis with locked nucleic acid probes

Title
Genotyping of velvet antlers for identification of country of origin using mitochondrial DNA and fluorescence melting curve analysis with locked nucleic acid probes
Author
황승용
Keywords
Cervus elaphus; chronic wasting disease; country of origin; fluorescence melting curve analysis; locked nucleic acids
Issue Date
2016-07
Publisher
TAYLOR & FRANCIS LTD
Citation
MITOCHONDRIAL DNA (2016), v. 27, NO. 4, Page. 2641-2644
Abstract
Velvet antlers are used medicinally in Asia and possess various therapeutic effects. Prices are set according to the country of origin, which is unidentifiable to the naked eye, and therefore counterfeiting is prevalent. Additionally, antlers of the Canadian elk, which can generate chronic wasting disease, are prevalently smuggled and distributed in the market. Thus, a method for identifying the country of origin of velvet antlers was developed, using polymorphisms in mitochondrial DNA, fluorescence melting curve analysis and analysis of locked nucleic acids (LNA). This combined method is capable of identifying five genotypes of velvet antlers in a single experiment using two probes. It also has advantages in multiplexing, simplicity and efficiency in genotyping, when compared to real-time PCR or microarrays. The developed method can be used to improve identification rates in the velvet antler market and, by extension, research based on polymorphisms in DNA sequences.
URI
https://www.tandfonline.com/doi/full/10.3109/19401736.2015.1041133http://repository.hanyang.ac.kr/handle/20.500.11754/74532
ISSN
2470-1394; 2470-1408
DOI
10.3109/19401736.2015.1041133
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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