Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-07-25T04:45:20Z | - |
dc.date.available | 2018-07-25T04:45:20Z | - |
dc.date.issued | 2011-06 | - |
dc.identifier.citation | Tissue Engineering Part A; New Rochelle Vol. 17, Iss. 17-18, (Sep 2011): 2153-64. | en_US |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://search.proquest.com/openview/85784a4568dbf81c4002f574f8b068af/1?pq-origsite=gscholar&cbl=40309 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/72875 | - |
dc.description.abstract | Bone morphogenetic proteins (BMPs) are the most potent osteoinductive growth factors. BMP-2 is clinically used for spine fusion and bone fracture healing. Commercially available BMP-2 uses a type I collagen scaffold as a carrier, but it only releases BMP-2 for a short period of time, which may release the bone formation efficacy. In the present study, we hypothesize that apatite coating of a collagen scaffold increases the release period as well as the osteogenic efficacy of BMP-2. Apatite coating was achieved by incubating collagen scaffolds in simulated body fluids (SBFs). Apatite coating on collagen scaffolds was confirmed by X-ray diffraction, electron spectroscopy for chemical analysis, attenuated total reflectance-Fourier transform infrared spectroscopy, and scanning electron microscopy. The rate and period of BMP-2 release from apatite-coated collagen scaffolds varied depending on the concentration of SBFs used. The 5x and 10x SBF apatite-coated collagen scaffolds released 91.8%+/- 11.5% and 82.2%+/- 13.1% of their loaded BMP-2 over 13 days in vitro, respectively, whereas noncoated collagen scaffold released 98.3%+/- 2.2% over the initial one day. BMP-2 released from apatite-coated collagen scaffold significantly increased the alkaline phosphatase activity of cultured osteoblasts, compared with BMP-2 released from noncoated collagen scaffold. Computed tomography and histomorphometry showed that BMP-2 delivery using apatite-coated collagen scaffolds resulted in 2.5-fold higher bone formation volume and 4.0-fold higher bone formation area than BMP-2 delivery using noncoated collagen scaffolds. This study shows that simple apatite coating of a collagen scaffold results in a BMP-2 carrier that renders long-term release of BMP-2 and dramatically enhances osteogenic efficacy. | en_US |
dc.description.sponsorship | This study was supported by a grant (2009-0080769) from the National Research Foundation of Korea, a grant (A100443) from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | MARY ANN LEIBERT INC | en_US |
dc.title | Apatite-Coated Collagen Scaffold for Bone Morphogenetic Protein-2 Delivery | en_US |
dc.type | Article | en_US |
dc.relation.no | 17-18 | - |
dc.relation.volume | 17 | - |
dc.identifier.doi | 10.1089/ten.tea.2010.0702 | - |
dc.relation.page | 2153-2164 | - |
dc.relation.journal | TISSUE ENGINEERING PART A | - |
dc.contributor.googleauthor | Yang, Hee Seok | - |
dc.contributor.googleauthor | La, Wan-Geun | - |
dc.contributor.googleauthor | Bhang, Suk Ho | - |
dc.contributor.googleauthor | Lee, Tae-Jin | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | Kim, Byung-Soo | - |
dc.relation.code | 2011216617 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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