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Apatite-Coated Collagen Scaffold for Bone Morphogenetic Protein-2 Delivery

Title
Apatite-Coated Collagen Scaffold for Bone Morphogenetic Protein-2 Delivery
Author
이민형
Issue Date
2011-06
Publisher
MARY ANN LEIBERT INC
Citation
Tissue Engineering Part A; New Rochelle Vol. 17, Iss. 17-18, (Sep 2011): 2153-64.
Abstract
Bone morphogenetic proteins (BMPs) are the most potent osteoinductive growth factors. BMP-2 is clinically used for spine fusion and bone fracture healing. Commercially available BMP-2 uses a type I collagen scaffold as a carrier, but it only releases BMP-2 for a short period of time, which may release the bone formation efficacy. In the present study, we hypothesize that apatite coating of a collagen scaffold increases the release period as well as the osteogenic efficacy of BMP-2. Apatite coating was achieved by incubating collagen scaffolds in simulated body fluids (SBFs). Apatite coating on collagen scaffolds was confirmed by X-ray diffraction, electron spectroscopy for chemical analysis, attenuated total reflectance-Fourier transform infrared spectroscopy, and scanning electron microscopy. The rate and period of BMP-2 release from apatite-coated collagen scaffolds varied depending on the concentration of SBFs used. The 5x and 10x SBF apatite-coated collagen scaffolds released 91.8%+/- 11.5% and 82.2%+/- 13.1% of their loaded BMP-2 over 13 days in vitro, respectively, whereas noncoated collagen scaffold released 98.3%+/- 2.2% over the initial one day. BMP-2 released from apatite-coated collagen scaffold significantly increased the alkaline phosphatase activity of cultured osteoblasts, compared with BMP-2 released from noncoated collagen scaffold. Computed tomography and histomorphometry showed that BMP-2 delivery using apatite-coated collagen scaffolds resulted in 2.5-fold higher bone formation volume and 4.0-fold higher bone formation area than BMP-2 delivery using noncoated collagen scaffolds. This study shows that simple apatite coating of a collagen scaffold results in a BMP-2 carrier that renders long-term release of BMP-2 and dramatically enhances osteogenic efficacy.
URI
https://search.proquest.com/openview/85784a4568dbf81c4002f574f8b068af/1?pq-origsite=gscholar&cbl=40309http://repository.hanyang.ac.kr/handle/20.500.11754/72875
ISSN
1937-3341
DOI
10.1089/ten.tea.2010.0702
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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