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Lactobacillus casei prevents impaired barrier function in intestinal epithelial cells

Title
Lactobacillus casei prevents impaired barrier function in intestinal epithelial cells
Author
김용석
Keywords
Lactobacillus casei; epithelial barrier function; Caco-2
Issue Date
2011-01
Publisher
WILEY-BLACKWELL PUBLISHING, INC, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
Citation
APMIS(Acta Pathologica Microbiologica et Immunologica Scandinavica),Vol.119 No.1 [2011],49-56
Abstract
The exact effect of probiotics on epithelial barrier function is not well understood. The aims of this study were to evaluate cytokine-induced epithelial barrier dysfunction in intestinal epithelial cells (IECs) and to study the role of probiotics in the prevention of epithelial barrier dysfunction. Caco-2 cells grown on transwell chambers were stimulated with tumor necrosis factor (TNF)-alpha or interferon (IFN)-gamma basolaterally. Probiotic, Lactobacillus casei, was added 1 h before cytokine stimulation. MAPK inhibitors were added 15 min before L. casei stimulation. The electrical resistance and paracellular permeability of Caco-2 monolayers were measured. Distribution of zonula occludens (ZO)-1 protein was assessed by immunofluorescence, and Western blot analyses for ZO-1, p-Akt, and toll-like receptor (TLR) 2 were performed. Both TNF-alpha and IFN-gamma stimulation on Caco-2 cells decreased transepithelial resistance (TER), increased epithelial permeability, and decreased ZO-1 expression of Caco-2 cells. In contrast, pretreatment of L. casei reversed the cytokine-induced dysfunction of TER, epithelial permeability, and ZO-1 expression. Reversal of cytokine-induced dysfunction of TER and intestinal permeability by L. casei was abrogated with MAPK inhibitor treatment. Lactobacillus casei stimulation on Caco-2 cells increased TLR2 and p-Akt expression. Probiotic, L. casei, prevents cytokine-induced epithelial barrier dysfunctions in IECs.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0463.2010.02691.xhttp://repository.hanyang.ac.kr/handle/20.500.11754/72769
ISSN
0903-4641
DOI
10.1111/j.1600-0463.2010.02691.x
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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