Altered Expression of Tight Junction Proteins in Cyclosporine Nephrotoxicity
- Title
- Altered Expression of Tight Junction Proteins in Cyclosporine Nephrotoxicity
- Author
- 이창화
- Keywords
- Cyclosporine nephrotoxicity; Paracellular permeability; Sodium chloride symporter; Tight junction proteins; WNK4 protein
- Issue Date
- 2011-01
- Publisher
- S. Karger AG
- Citation
- American journal of nephrology v.33 no.1,
- Abstract
- Background/Aims: The increased permeability of chloride in the distal cortical nephron in cyclosporine nephrotoxicity may involve the transcellular pathway mediated by the thiazide-sensitive Na + -Cl - cotransporter and/or the paracellular pathway mediated by the tight junctions (TJs). Methods: Cyclosporine was subcutaneously administered to Sprague-Dawley rats for 6 (7.5 mg/kg body weight) and 2 (25 mg/kg body weight) weeks, and immunoblot analysis and immunohistochemistry were carried out from the kidneys. Electrically tight epithelial Madin-Darby canine kidney (MDCK) I cells were exposed to cyclosporine for 72 h to measure changes in transepithelial electrical resistance (ΔTER). Results: Cyclosporine treatment induced a decrease in Na + -Cl - cotransporter in rat renal cortex. WNK4 protein was increased in both rat kidneys and MDCK I cells. Occludin was also increased in rat kidneys and MDCK I cells exposed to 100 ng/ml cyclosporine. In contrast, cyclosporine treatment induced a decrease in zonula occludens 1 protein abundance and no changes in claudin-1 and claudin-4 in both rat kidneys and MDCK I cells. As a measure of the barrier to small ions, ΔTER of MDCK monolayers was decreased by 100 ng/ml cyclosporine. Conclusion: Renal TJ proteins are affected by cyclosporine treatment. Changes in TJ protein assembly induced by altered expression of WNK4, occludin, and zonula occludens 1 may affect paracellular permeability.
- URI
- https://www.karger.com/Article/Abstract/322445https://repository.hanyang.ac.kr/handle/20.500.11754/72740
- ISSN
- 0250-8095; 1421-9670
- DOI
- 10.1159/000322445
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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