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Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B

Title
Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B
Author
주재범
Keywords
ENHANCED RAMAN-SCATTERING; IMMUNOCHROMATOGRAPHIC ASSAY; GOLD NANOPARTICLES; VISUAL DETECTION; COLLOIDAL GOLD; CANCER MARKERS; QUANTUM DOTS; BIOSENSOR; LABEL; IMMUNOSENSOR
Issue Date
2016-06
Publisher
ROYAL SOC CHEMISTRY
Citation
NANOSCALE, v. 8, NO 22, Page. 11418-11425
Abstract
A novel surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFA) biosensor was developed to resolve problems associated with conventional LFA strips (e.g., limits in quantitative analysis and low sensitivity). In our SERS-based biosensor, Raman reporter-labeled hollow gold nanospheres (HGNs) were used as SERS detection probes instead of gold nanoparticles. With the proposed SERS-based LFA strip, the presence of a target antigen can be identified through a colour change in the test zone. Furthermore, highly sensitive quantitative evaluation is possible by measuring SERS signals from the test zone. To verify the feasibility of the SERS-based LFA strip platform, an immunoassay of staphylococcal enterotoxin B (SEB) was performed as a model reaction. The limit of detection (LOD) for SEB, as determined with the SERS-based LFA strip, was estimated to be 0.001 ng mL(-1). This value is approximately three orders of magnitude more sensitive than that achieved with the corresponding ELISA-based method. The proposed SERS-based LFA strip sensor shows significant potential for the rapid and sensitive detection of target markers in a simplified manner.
URI
http://pubs.rsc.org/en/Content/ArticleLanding/2016/NR/C5NR07243C#!divAbstracthttp://repository.hanyang.ac.kr/handle/20.500.11754/72601
ISSN
2040-3364; 2040-3372
DOI
10.1039/c5nr07243c
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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