Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer
- Title
- Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer
- Author
- 이수재
- Keywords
- POLYCYCLIC AROMATIC-HYDROCARBONS; CRUDE-OIL; BRACHIONUS-KOREANUS; MONOGONONT ROTIFER; CYTOCHROME-P450 CYP; OXIDATIVE STRESS; PROTEIN-KINASES; DISPERSED OIL; TOXICITY; SPILL
- Issue Date
- 2016-06
- Publisher
- AMER CHEMICAL SOC
- Citation
- ENVIRONMENTAL SCIENCE & TECHNOLOGY, v. 50, NO 11, Page. 6025-6035
- Abstract
- To examine the deleterious effects of the water accommodated fraction (WAF) of crude oil, the growth curve, fecundity, and lifespan of the monogonont rotifer (Brachionus koreanus) were measured for 24 h in response to three different doses (0.2X, 0.4X, and 0.8X) of WAFs. A higher dose of WAFs significantly reduced the fecundity and lifespan. A rotifer 32K microarray chip showed that the Bk-CYP3045C1 gene had the highest expression. Of the 25 entire CYP genes, the Bk-CYP3045C1 gene showed a significant expression for different doses and times in response to WAFs and chemical components of WAFs (naphthalene and phenanthrene); also, glutathione S-transferase genes, ABC transporter, and other genes showed dose responses upon exposure to 80% WAF over time. Different doses of WAFs increased the oxidative stress with an induction of reactive oxygen species (ROS) and a depletion of glutathione (GSH). Exposure to WAFs did not show toxic effects on survivability in B. koreanus; however, toxicity to WAFs was shown when piperonyl butoxide, a potent inhibitor of cytochrome P450 (CYP) enzymes, was added. This toxicity was dose-dependent. After WAFs exposure, PERK was activated over time in response to WAFs, which suggests that WAFs can be activated by the p-ERK signaling pathway.
- URI
- https://pubs.acs.org/doi/10.1021/acs.est.6b01306https://repository.hanyang.ac.kr/handle/20.500.11754/72467
- ISSN
- 0013-936X; 1520-5851
- DOI
- 10.1021/acs.est.6b01306
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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