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Failure of a patient-derived xenograft for brain tumor model prepared by implantation of tissue fragments

Title
Failure of a patient-derived xenograft for brain tumor model prepared by implantation of tissue fragments
Author
이수재
Keywords
Glioblastoma; Model failure; Patient-derived xenograft; Primitive neuro-ectodermal tumor; Tissue fragment
Issue Date
2016-06
Publisher
BIOMED CENTRAL LTD
Citation
CANCER CELL INTERNATIONAL, v. 16, Page. 1-6
Abstract
Background: With the continuing development of new anti-cancer drugs comes a need for preclinical experimental models capable of predicting the clinical activity of these novel agents in cancer patients. However existing models have a limited ability to recapitulate the clinical characteristics and associated drug sensitivity of tumors. Among the more promising approaches for improving preclinical models is direct implantation of patient-derived tumor tissue into immunocompromised mice, such as athymic nude or non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice. In the current study, we attempted to develop patient-derived xenograft (PDX) models using tissue fragments from surgical samples of brain tumors. Methods: In this approach, tiny tissue fragments of tumors were biopsied from eight brain tumor patients-seven glioblastoma patients and one primitive neuroectodermal tumor patient. Two administration methods-a cut-down syringe and a pipette-were used to implant tissue fragments from each patient into the brains of athymic nude mice. Results: In contrast to previous reports, and contrary to our expectations, we found that none of these fragments from brain tumor biopsies resulted in the successful establishment of xenograft tumors. Conclusions: These results suggest that fragments of surgical specimens from brain tumor patients are unsuitable for implementation of brain tumor PDX models, and instead recommend other in vivo testing platforms for brain tumors, such as cell-based brain tumor models.
URI
https://cancerci.biomedcentral.com/articles/10.1186/s12935-016-0319-0https://repository.hanyang.ac.kr/handle/20.500.11754/72426
ISSN
1475-2867
DOI
10.1186/s12935-016-0319-0
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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