Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-06-18T05:09:57Z | - |
dc.date.available | 2018-06-18T05:09:57Z | - |
dc.date.issued | 2016-06 | - |
dc.identifier.citation | PHARMACEUTICAL RESEARCH, v. 33, NO 9, Page. 2250-2258 | en_US |
dc.identifier.issn | 0724-8741 | - |
dc.identifier.issn | 1573-904X | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs11095-016-1962-9 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/72120 | - |
dc.description.abstract | To reduce side effects due to non-specific expression, the heme oxygenase-1 (HO-1) gene under control of a hypoxia-inducible erythropoietin (Epo) enhancer (pEpo-SV-HO-1) was developed for site-specific gene therapy of ischemic stroke. pEpo-SV-HO-1 was constructed by insertion of the Epo enhancer into pSV-HO-1. Dexamethasone-conjugated polyamidoamine (PAMAM-Dexa) was used as a gene carrier. In vitro transfection assays were performed in the Neuro2A cells. In vivo efficacy of pEpo-SV-HO-1 was evaluated in the transient middle cerebral artery occlusion (MCAO) model. In vitro transfection assay with the PAMAM-Dexa/pEpo-SV-HO-1 complex showed that pEpo-SV-HO-1 had higher HO-1 gene expression than pSV-HO-1 under hypoxia. In addition, pEpo-SV-HO-1 reduced the level of apoptosis more efficiently than pSV-HO-1 in Neuro2A cells under hypoxia. For in vivo evaluation, the PAMAM-Dexa/pEpo-SV-HO-1 complex was injected into the ischemic brain of the transient MCAO model. pEpo-SV-HO-1 increased HO-1 expression and reduced the number of apoptotic cells in the ischemic brain, compared with the pSV-HO-1 injection group. As a result, the infarct volume was more efficiently decreased by pEpo-SV-HO-1 than by pSV-HO-1. pEpo-SV-HO-1 induced HO-1 gene expression and therapeutic effect in the ischemic brain. Therefore, pEpo-SV-HO-1 may be useful for site-specific gene therapy of ischemic stroke. | en_US |
dc.description.sponsorship | This work was supported by a grant from the National Research Foundation of Korea, funded by the Ministry of Science, ICT and Future Planning (NRF-2013R1A1A2059236). | en_US |
dc.language.iso | en | en_US |
dc.publisher | SPRINGER/PLENUM PUBLISHERS | en_US |
dc.subject | gene delivery | en_US |
dc.subject | heme oxygenase-1 | en_US |
dc.subject | hypoxia-inducible gene | en_US |
dc.subject | ischemic stroke | en_US |
dc.subject | site-specific gene therapy | en_US |
dc.title | Delivery of Hypoxia-Inducible Heme Oxygenase-1 Gene for Site-Specific Gene Therapy in the Ischemic Stroke Animal Model | en_US |
dc.type | Article | en_US |
dc.relation.no | 9 | - |
dc.relation.volume | 33 | - |
dc.identifier.doi | 10.1007/s11095-016-1962-9 | - |
dc.relation.page | 2250-2258 | - |
dc.relation.journal | PHARMACEUTICAL RESEARCH | - |
dc.contributor.googleauthor | Choi, Manbok | - |
dc.contributor.googleauthor | Oh, Jungju | - |
dc.contributor.googleauthor | Rhim, Taiyoun | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.relation.code | 2016000646 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.