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Pre-elafin is Involved in Ultraviolet-induced Keratinocyte Apoptosis via Pro-caspase-3 Activation Associated with Cystatin-A Downregulation

Title
Pre-elafin is Involved in Ultraviolet-induced Keratinocyte Apoptosis via Pro-caspase-3 Activation Associated with Cystatin-A Downregulation
Author
배옥남
Keywords
UV; keratinocyte apoptosis; pre-elafin; cellular location; cystatin-A; pro-caspase-3
Issue Date
2017-05
Publisher
ACTA DERMATO-VENEREOLOGICA
Citation
ACTA DERMATO-VENEREOLOGICA, v. 97, No. 5, Page. 578-585
Abstract
Pre-elafin controls keratinocyte integrity via cornified envelope formation and inhibition of desquamation, but its role in ultraviolet (UV)-induced keratinocyte apoptosis is unknown. This study examined the role of pre-elafin in volunteer skin samples and primary cultured normal human keratinocytes irradiated with phototoxic doses of UVA/narrow-band UVB, and in keratinocytes with pre-elafin overexpression/knockdown, under conditions of low and high calcium. Phototoxic doses of UV increased pre-elafin mRNA and protein expression in inverse proportion to keratinocyte survival. Pre-elafin overexpression under conditions of low calcium, which, in contrast to conditions of high calcium, was localized to the cytoplasm, increased keratinocyte apoptosis, whereas knockdown inhibited UV-induced apoptosis. Pre-elafin was co-localized with, but not bound to, cleaved caspase-3. Pre-elafin reduced cystatin-A expression, which was bound to pro-caspase-3. In conclusion, UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation.
URI
http://www.ingentaconnect.com/contentone/mjl/adv/2017/00000097/00000005/art00004http://repository.hanyang.ac.kr/handle/20.500.11754/72084
ISSN
0001-5555; 1651-2057
DOI
10.2340/00015555-2621
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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