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Efficient approach to purification of octaphlorethol A from brown seaweed, Ishige foliacea by centrifugal partition chromatography

Title
Efficient approach to purification of octaphlorethol A from brown seaweed, Ishige foliacea by centrifugal partition chromatography
Author
김철영
Keywords
Antioxidant effect; Ishige foliacea; ABTS(+) on-line HPLC; Centrifugal partition chromatography; Octaphlorethol A; CELLS; ALGA; ECKLONIA-CAVA; PREPARATIVE ISOLATION; OXIDATIVE STRESS; ANTIOXIDANT ACTIVITIES; IN-VITRO; ONE-STEP; PHLOROTANNINS; ZEBRAFISH
Issue Date
2017-03
Publisher
ELSEVIER SCIENCE BV
Citation
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 22, Page. 87-92
Abstract
The brown seaweed, Ishige foliacea is distributed along the coast of Jeju Island. The main compound that this seaweed produces, octaphlorethol A (OPA), has been recently reported to exhibit strong antidiabetic andwhitening activities. However, obtaining pure OPA requires repetitive complex processes and its antioxidant effect in vivo has not been demonstrated. Therefore, we devised a method to efficiently detect and isolate OPA from I. foliacea using 2.2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(+)) on-line HPLC and centrifu-gal partition chromatography (CPC), respectively. And we evaluated its antioxidative properties using zebrafish as an in vivo model system. OPA among compounds from I. foliacea ethyl acetate (EtOAc) fraction had the lowest absorbance in ABTS(+) on-line HPLC. A total of 11.2 mg OPA was rapidly isolated from the EtOAc fraction (500mg) of I. foliacea extract by using CPC with a two-phase solvent system. In particular, OPA strongly inhibited reactive oxygen species and lipid peroxidation in 2.2'-azobis (2-amidinopropane) dihydro-chloride-induced zebrafish embryos. (C) 2016 Published by Elsevier B.V.
URI
https://www.sciencedirect.com/science/article/pii/S2211926416307329http://repository.hanyang.ac.kr/handle/20.500.11754/72001
ISSN
2211-9264
DOI
10.1016/j.algal.2016.12.006
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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