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Enhancing the in vitro anticancer activity of albendazole incorporated into chitosan-coated PLGA nanoparticles

Title
Enhancing the in vitro anticancer activity of albendazole incorporated into chitosan-coated PLGA nanoparticles
Author
김진기
Keywords
MICROPARTICLES; Mucoadhesion; Albendazole; PLGA Nanoparticle; Chitosan; Anticancer activity; POLYSACCHARIDE; SOLID LIPID NANOPARTICLES; ORAL INSULIN DELIVERY; DRUG-DELIVERY; RELEASE; BIOAVAILABILITY; ABSORPTION; POLYMERS; PEPTIDE
Issue Date
2017-03
Publisher
ELSEVIER SCI LTD
Citation
CARBOHYDRATE POLYMERS, v. 159, Page. 39-47
Abstract
To improve the solubility and anticancer activity of albendazole (ABZ), chitosan (CS)-coated poly-dl-lactic-co-glycolic acid (PLGA) nanoparticles were developed. CS was used to coat ABZ-loaded PLGA nanoparticles to enhance both mucoadhesiveness and colloidal stability. CS-coated PLGA nanoparticles were prepared by suspending the nanoparticles in CS solution after solvent diffusion. The CS-coated PLGA nanoparticles were characterized, and ABZ release was studied in vitro from various formulations. The mucoadhesive properties and in vitro anticancer activities of CS-coated PLGA nanoparticles were investigated by measurement of zeta potentials and the MTT assay, respectively. Spherical nanoparticles below 500 nm in diameter were successfully prepared; the particle size distribution was narrow. Complete encapsulation of ABZ in CS-coated PLGA nanoparticles was confirmed by SEM, FTIR, DSC, and XRD. The particle sizes of CS-coated PLGA nanoparticles were in the range of 260-480 nm; the encapsulation efficiency was 43.4-54.6%; and the yield 58.5-67.8%. The zeta potential of CS-coated nanoparticles was above +27 mV and stability was maintained for 4 weeks. At pH 7.4, the in vitro release of ABZ from nanoparticles (P188-5) was 200-fold higher than that from untreated ABZ; this persisted for 12 h. Moreover, ABZ release from CS-coated PLGA nanoparticles (P188-CS0.5) was 1.5-fold higher than that from untreated ABZ at pH 1.2. Additionally, the ABZ-loaded CS-coated nanoparticles exhibited superior mucoadhesion and improved cytotoxicity. The results show that CS coating of PLGA nanoparticles may improve the anticancer effect and the mucoadhesive properties of ABZ-loaded nanoparticles. (C) 2016 Elsevier Ltd. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0144861716313820http://repository.hanyang.ac.kr/handle/20.500.11754/72000
ISSN
0144-8617
DOI
10.1016/j.carbpol.2016.12.009
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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