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Silver nanoparticle-embedded graphene oxide-methotrexate for targeted cancer treatment

Title
Silver nanoparticle-embedded graphene oxide-methotrexate for targeted cancer treatment
Author
최한곤
Keywords
Graphene oxide; Silver nanoparticles; Methotrexate; Combined chemotherapy; Reactive oxygen species; LIQUID-CRYSTALLINE NANOPARTICLES; CHEMO-PHOTOTHERMAL THERAPY; CARBON-BASED NANOMATERIALS; ANTIBACTERIAL ACTIVITY; DELIVERY; CELLS; NANOCOMPOSITE; OPPORTUNITIES; CYTOTOXICITY; COMBINATION
Issue Date
2017-03
Publisher
ELSEVIER SCIENCE BV
Citation
COLLOIDS AND SURFACES B-BIOINTERFACES, v. 153, Page. 95-103
Abstract
Combination therapies are widely investigated cancer treatment modalities. Carbon based systems such as graphene oxide (GO), plasmonic nanoparticles such as silver nanoparticles (AgNPs), and the folate analog, methotrexate (MTX), have been separately studied for their potential anticancer effects. In this study, we combined these systems to develop AgNPs-embedded GO with conjugated MTX (MTX-GO/AgNPs) and studied their folate receptor-targeted anticancer effects. Results revealed successful formation of AgNPs on GO along with MTX conjugation as suggested by UV/visible, TEM, AFM, FTIR, and XRD analysis. Folate receptor-positive MCF-7 cells were more prone to cytotoxic effects of MTX-GO/AgNPs compared to folate receptor-negative HepG2 cells. Folic acid analog MTX interacts with folate receptors expressed in MCF-7 cells, improving cellular uptake and subsequent anticancer effects of the system. Importantly, AgNPs enhanced the total ROS production within the treated cells leading to improve cellular apoptosis, as evidenced by western blot. Moreover, near infrared (NIR)-induced photothermal effects of GO improved the anticancer activity of the system. Therefore, the combinational therapy system MTX-GO/AgNPs can be potentially applied for effective folate receptor-targeted treatment of cancers. (C) 2017 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S092777651730084Xhttp://repository.hanyang.ac.kr/handle/20.500.11754/71819
ISSN
0927-7765; 1873-4367
DOI
10.1016/j.colsurfb.2017.02.012
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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