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Gomisin N Inhibits Melanogenesis through Regulating the PI3K/Akt and MAPK/ERK Signaling Pathways in Melanocytes

Title
Gomisin N Inhibits Melanogenesis through Regulating the PI3K/Akt and MAPK/ERK Signaling Pathways in Melanocytes
Author
김철영
Keywords
Schisandra chinensis; Gomisin N; lignan; melanogenesis; skin whitening; DECREASES MELANIN SYNTHESIS; TRANSCRIPTION FACTOR; SKIN PIGMENTATION; L-TYROSINE; CELLS; MICROPHTHALMIA; MITF; EXPRESSION; EXTRACT; DISEASE
Issue Date
2017-02
Publisher
MDPI AG
Citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 18, No. 2, Article no. 471
Abstract
Gomisin N, one of the lignan compounds found in Schisandra chinensis has been shown to possess anti-oxidative, anti-tumorigenic, and anti-inflammatory activities in various studies. Here we report, for the first time, the anti-melenogenic efficacy of Gomisin N in mammalian cells as well as in zebrafish embryos. Gomisin N significantly reduced the melanin content without cellular toxicity. Although it was not capable of modulating the catalytic activity of mushroom tyrosinase in vitro, Gomisin N downregulated the expression levels of key proteins that function in melanogenesis. Gomisin N downregulated melanocortin 1 receptor (MC1R), adenylyl cyclase 2, microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2). In addition, Gomisin N-treated Melan-A cells exhibited increased p-Akt and p-ERK levels, which implies that the activation of the PI3K/Akt and MAPK/ERK pathways may function to inhibit melanogenesis. We also validated that Gomisin N reduced melanin production by repressing the expression of MITF, tyrosinase, TRP-1, and TRP-2 in mouse and human cells as well as in developing zebrafish embryos. Collectively, we conclude that Gomisin N inhibits melanin synthesis by repressing the expression of MITF and melanogenic enzymes, probably through modulating the PI3K/Akt and MAPK/ERK pathways.
URI
http://www.mdpi.com/1422-0067/18/2/471/htmhttp://repository.hanyang.ac.kr/handle/20.500.11754/71695
ISSN
1422-0067
DOI
10.3390/ijms18020471
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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