Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis

Title
Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis
Author
류재숙
Keywords
Trichomonas vaginalis; trichomoniasis; PCR; multiplex PCR; loop-mediated isothermal amplification
Issue Date
2016-06
Publisher
KOREAN SOC PARASITOLOGY
Citation
KOREAN JOURNAL OF PARASITOLOGY, v. 54, NO 3, Page. 329-334
Abstract
Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus, emphasizes the importance of improved access to early and accurate detection of T. vaginalis. In this study, a rapid and efficient loop-mediated isothermal amplification-based method for the detection of T. vaginalis was developed and validated, using vaginal swab specimens from subjects suspected to have trichomoniasis. The LAMP assay targeting the actin gene was highly sensitive with detection limits of 1 trichomonad and 1 pg of T. vaginalis DNA per reaction, and specifically amplified the target gene only from T. vaginalis. Validation of this assay showed that it had the highest sensitivity and better agreement with PCR (used as the gold standard) compared to microscopy and multiplex PCR. This study showed that the LAMP assay, targeting the actin gene, could be used to diagnose early infections of T. vaginalis. Thus, we have provided an alternative molecular diagnostic tool and a point-of-care test that may help to prevent trichomoniasis transmission and associated complications.
URI
http://parasitol.kr/journal/view.php?doi=10.3347/kjp.2016.54.3.329http://repository.hanyang.ac.kr/handle/20.500.11754/71655
ISSN
0023-4001; 1738-0006
DOI
https://doi.org/10.3347/kjp.2016.54.3.329
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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