Magnetic bead-based nucleic acid purification kit: Clinical application and performance evaluation in stool specimens
- Title
- Magnetic bead-based nucleic acid purification kit: Clinical application and performance evaluation in stool specimens
- Author
- 황승용
- Keywords
- Nucleic acid extraction; Stool; Clostridium difficile; Rotavirus
- Issue Date
- 2016-05
- Publisher
- ELSEVIER SCIENCE BV
- Citation
- JOURNAL OF MICROBIOLOGICAL METHODS, v. 124, Page. 62-68
- Abstract
- Two different methods - the semi-automated magnetic bead-based kit (SK, Stool DNA/RNA Purification kit) and the manual membrane column-based kit (QS, QlAamp (R) DNA Stool Mini kit) - for purifying nucleic acids from clinical stool samples were compared and evaluated. The SK kit was more user-friendly than QS due to the reduced manual processing, partial automation, and short turnaround time with half cost. Furthermore, SK produced high yields in both DNA and RNA extractions but poor purity in RNA extraction. In the assessment of rotavirus and Clostridium difficile infection, both kits had equivalent or more sensitive performance compared with the standard method. Although SK showed some interference and inhibition in nucleic acid extraction, the performance, including the repeatability, linearity, analytical sensitivity, and matrix effect, was sufficient for routine clinical use. (C) 2016 Elsevier B.V. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S0167701216300458?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/71513
- ISSN
- 0167-7012; 1872-8359
- DOI
- 10.1016/j.mimet.2016.03.011
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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