Accessible Mannitol-Based Amphiphiles (MNAs) for Membrane Protein Solubilisation and Stabilisation

Title
Accessible Mannitol-Based Amphiphiles (MNAs) for Membrane Protein Solubilisation and Stabilisation
Author
채필석
Keywords
amphiphile design; electron microscopy; membrane proteins; novel detergents; protein stabilization
Issue Date
2016-05
Publisher
WILEY-V C H VERLAG GMBH
Citation
CHEMISTRY-A EUROPEAN JOURNAL, v. 22, NO 21, Page. 7068-7073
Abstract
Integral membrane proteins are amphipathic molecules crucial for all cellular life. The structural study of these macromolecules starts with protein extraction from the native membranes, followed by purification and crystallisation. Detergents are essential tools for these processes, but detergent-solubilised membrane proteins often denature and aggregate, resulting in loss of both structure and function. In this study, a novel class of agents, designated mannitol-based amphiphiles (MNAs), were prepared and characterised for their ability to solubilise and stabilise membrane proteins. Some of MNAs conferred enhanced stability to four membrane proteins including a G protein-coupled receptor (GPCR), the beta(2) adrenergic receptor (beta(2)AR), compared to both n-dodecyl-D-maltoside (DDM) and the other MNAs. These agents were also better than DDM for electron microscopy analysis of the beta(2)AR. The ease of preparation together with the enhanced membrane protein stabilisation efficacy demonstrates the value of these agents for future membrane protein research.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201600533http://repository.hanyang.ac.kr/handle/20.500.11754/71401
ISSN
0947-6539; 1521-3765
DOI
10.1002/chem.201600533
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE