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Global Analyses of the Effect of Different Cellular Contexts on MicroRNA Targeting

Title
Global Analyses of the Effect of Different Cellular Contexts on MicroRNA Targeting
Author
남진우
Issue Date
2014-03
Publisher
Elsevier Science B.V
Citation
Molecular Cell, 2014, 53(6), P.1031-1043
Abstract
MicroRNA (miRNA) regulation clearly impacts animal development, but the extent to which development-with its resulting diversity of cellular contexts-impacts miRNA regulation is unclear. Here, we compared cohorts of genes repressed by the same miRNAs in different cell lines and tissues and found that target repertoires were largely unaffected, with secondary effects explaining most of the differential responses detected. Outliers resulting from differential direct targeting were often attributable to alternative 3' UTR isoform usage that modulated the presence of miRNA sites. More inclusive examination of alternative 3' UTR isoforms revealed that they influence similar to 10% of predicted targets when comparing any two cell types. Indeed, considering alternative 3' UTR isoform usage improved prediction of targeting efficacy significantly beyond the improvements observed when considering constitutive isoform usage. Thus, although miRNA targeting is remarkably consistent in different cell types, considering the 3' UTR landscape helps predict targeting efficacy and explain differential regulation that is observed.
URI
https://www.cell.com/molecular-cell/fulltext/S1097-2765(14)00129-4http://repository.hanyang.ac.kr/handle/20.500.11754/71198
ISSN
1097-2765
DOI
10.1016/j.molcel.2014.02.013
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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