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Quantitative Analysis of H5N1 DNA Hybridization on Nanowell Array Electrode

Title
Quantitative Analysis of H5N1 DNA Hybridization on Nanowell Array Electrode
Author
박진구
Keywords
Nanowell Array Electrode; H5N1 DNA; Electrochemical Impedance Spectroscopy (EIS); Charge Transfer Resistance (R-ct)
Issue Date
2013-08
Publisher
AMER SCIENTIFIC PUBLISHERS, 26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA
Citation
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,권: 13 ,호: 8 , 페이지: 5245-5249
Abstract
A nanowell array electrode-based electrochemical quantitative system without amplification was developed and applied for the detection of H5N1 target DNA. An 18-mer probe was immobilized on a nanowell array electrode with a diameter of 500 nm, which was coated with streptavidin and a self-assembly monolayer (SAM). The surface properties of probe DNA hybridization with complementary target DNA were characterized using atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). The AFM image shows that the depth of nanowell was reduced from 200 nm to 15 nm due to the formation of a DNA hybridization complex on the streptavidin/SAM structure. Differences in charge transfer resistance (ΔRct) in EIS upon hybridization of the probe DNA with complementary target DNA were analyzed and used for the quantitation of H5N1 DNA. This approach shows that the quantitative analysis of H5N1 DNA ranging from 1 pM to 1 ?M DNA is possible on a nanowell array electrode.
URI
https://ir.ymlib.yonsei.ac.kr/bitstream/22282913/136487/1/TA01477.pdfhttp://repository.hanyang.ac.kr/handle/20.500.11754/70775
ISSN
1533-4880
DOI
10.1166/jnn.2013.7531
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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