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Human aortic endothelial cells compare favourably with macrophages for the study of anthrax toxins

Title
Human aortic endothelial cells compare favourably with macrophages for the study of anthrax toxins
Author
김성민
Keywords
anthrax toxins; protective antigen; lethal toxin; edema toxin; macrophages; human aortic endothelial cells
Issue Date
2013-07
Publisher
Inderscience Enterprises Ltd
Citation
INTERNATIONAL JOURNAL OF NANOTECHNOLOGY, 권: 10, 호: 8-9, 페이지: 756-770
Abstract
Anthrax is an infectious disease caused by Bacillus anthracis. Although B. anthracis is rare in the natural environment, anthrax spores can be produced in vitro and potentially used as a biological weapon. Thus, understanding the molecular pathogenesis of anthrax is a critical concern for national security. The aim of this study was to compare the effects of anthrax toxins, lethal toxin (LeTx), and edema toxin (EdTx) on human aortic endothelial cells (HAECs) and the J774A. 1 murine macrophage cell line. We analysed cell viability, adhesion, morphology, and lipopolysaccharide (LPS)-stimulated cytokine production after incubation of cells with varying concentrations of the toxins. Both LeTx and EdTx markedly inhibited LPS-induced transcription of tumour necrosis factor alpha (TNF-alpha), interleukin (IL)-1 alpha, and IL-6 in J774A. 1 cells. In contrast, EdTx synergised with LPS to increase the transcription of IL-6 and IL-8 in HAECs. We showed that HAECs are suitable for anthrax toxin research and express higher levels of the two anthrax toxin receptors - tumour endothelial marker 8 (TEM8/ANTXR1) and capillary morphogenesis protein 2 (CMG2/ANTXR2) - than do J774A. 1 cells. Collectively, our results suggest that HAECs may be superior to macrophages for the study of anthrax pathogenesis.
URI
https://www.researchgate.net/profile/Nirmal_Rajasekaran2/publication/250614935_Human_aortic_endothelial_cells_compare_favourably_with_macrophages_for_the_study_of_anthrax_toxins/links/55470cca0cf24107d3980ebe.pdfhttp://repository.hanyang.ac.kr/handle/20.500.11754/70742
ISSN
1475-7435
DOI
10.1504/IJNT.2013.054216
Appears in Collections:
COLLEGE OF ART AND PHYSICAL EDUCATION[S](예술·체육대학) > PHYSICAL EDUCATION(체육학과) > Articles
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