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Tentative identification of in vitro metabolites of 5-APDB, a synthetic benzofuran, by LC-Q/TOF-MS

Title
Tentative identification of in vitro metabolites of 5-APDB, a synthetic benzofuran, by LC-Q/TOF-MS
Author
유혜현
Keywords
5-APDB; Designer drug; Metabolite; LC-Q/TOP-MS; HUMAN LIVER-MICROSOMES; 5-(2-AMINOPROPYL)BENZOFURAN 5-APB; ILLEGAL PRODUCTS; DESIGNER DRUGS; GC-MS; INTOXICATION; ANALOGS; URINE; RAT
Issue Date
2016-10
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1033, Page. 296-300
Abstract
5-(2-Aminopropyl)-2,3-dihydrobenzofuran (5-APDB) is a designer drug of phenethylamine and amphetamine class. In this study, the in vitro metabolism of 5-APDB was investigated in rat and human liver microsomes and human hepatocytes to characterize its metabolites. 5-APDB was incubated with microsomes or hepatocytes, and the reaction mixture was analyzed using liquid chromatography-quadrupole time-of-flight with tandem mass spectrometry (LC-QJTOF-MS). 5-APDB was metabolized to yield three metabolites (M1, M2 and M3). These metabolites were structurally characterized on the basis of accurate mass analysis and MS/MS fragmentation patterns. Metabolite M1 and M2 were identified as hydroxylated metabolites in the benzofuran moiety; M3 was a reduced metabolite which may be generated from M1 or M2 via dehydration. These results provide evidence for the in vivo 5-APDB metabolism, and would be forensically useful for the detection of 5-APDB and its metabolites in biological samples. (C) 2016 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S1570023216307280http://repository.hanyang.ac.kr/handle/20.500.11754/70365
ISSN
1570-0232; 1873-376X
DOI
10.1016/j.jchromb.2016.08.043
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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