28-Day inhalation toxicity of graphene nanoplatelets in Sprague-Dawley rats

Title
28-Day inhalation toxicity of graphene nanoplatelets in Sprague-Dawley rats
Author
안강호
Keywords
Nano-structured materials; nanotoxicology; nanotechnology; particle toxicology; WALLED CARBON NANOTUBES; LUNG EPITHELIAL-CELLS; COMET ASSAY; EXPOSURE; POSTEXPOSURE; 13-WEEK; MWCNTS
Issue Date
2016-10
Publisher
TAYLOR & FRANCIS LTD
Citation
NANOTOXICOLOGY, v. 10, No. 7, Page. 891-901
Abstract
Graphene, a two-dimensional engineered nanomaterial, is now being used in many applications, such as electronics, biological engineering, filtration, lightweight and strong nanocomposite materials, and energy storage. However, there is a lack of information on the potential health effects of graphene in humans based on inhalation, the primary engineered nanomaterial exposure pathway in workplaces. Thus, an inhalation toxicology study of graphene was conducted using a nose-only inhalation system for 28 days (6h/day and 5 days/week) with male Sprague-Dawley rats that were then allowed to recover for 1-, 28-, and 90-day post-exposure period. Animals were separated into 4 groups (control, low, moderate, and high) with 15 male rats (5 rats per time point) in each group. The measured mass concentrations for the low, moderate, and high exposure groups were 0.12, 0.47, and 1.88mg/m(3), respectively, very close to target concentrations of 0.125, 0.5, and 2mg/m(3). Airborne graphene exposure was monitored using several real-time instrumentation over 10nm to 20m for size distribution and number concentration. The total and respirable elemental carbon concentrations were also measured using filter sampling. Graphene in the air and biological media was traced using transmission electron microscopy. In addition to mortality and clinical observations, the body weights and food consumption were recorded weekly. At the end of the study, the rats were subjected to a full necropsy, blood samples were collected for blood biochemical tests, and the organ weights were measured. No dose-dependent effects were recorded for the body weights, organ weights, bronchoalveolar lavage fluid inflammatory markers, and blood biochemical parameters at 1-day post-exposure and 28-day post-exposure. The inhaled graphenes were mostly ingested by macrophages. No distinct lung pathology was observed at the 1-, 28- and 90-day post-exposure. The inhaled graphene was translocated to lung lymph nodes. The results of this 28-day graphene inhalation study suggest low toxicity and a NOAEL of no less than 1.88mg/m(3).
URI
https://www.tandfonline.com/doi/abs/10.3109/17435390.2015.1133865http://repository.hanyang.ac.kr/handle/20.500.11754/70357
ISSN
1743-5390; 1743-5404
DOI
10.3109/17435390.2015.1133865
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MECHANICAL ENGINEERING(기계공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE