54 0

Fabrication of a hydrophobic/hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay

Title
Fabrication of a hydrophobic/hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay
Other Titles
hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay
Author
주재범
Keywords
Hydrophobic/hydrophilic pattern; SAM; Microarray; Immunoassay; Angiogenin
Issue Date
2012-05
Publisher
Korean Biochip Society
Citation
Biochip Journal, 2012, 6(1), P.10-16
Abstract
In this work, we report on a simple process for fabricating a hydrophobic/hydrophilic hybrid-patterned microarray chip for a fast and sensitive immunoassay. Two different types of self-assembled monolayers (SAMs) were used in the fabrication of hydrophilic well patterns and hydrophobic substrates. The hydrophilic/hydrophobic hybrid SAM pattern generates a clear-cut boundary between the sample and the background. A change in the precursor molecules allows for many different. types of SAMs to be employed in the fabrication process. Fluorescence image-based detection has previously been used for the quantitative immune-analysis of a specific cancer marker. Here, a titanium-coated glass substrate was utilized to suppress auto-fluorescence signals from substrate backgrounds. Angiogenin (ANG), a small polypeptide implicated in both angiogenesis and tumor growth, was used as a target cancer marker for its validation. Assay results demonstrate that the hybrid-patterned array chip yields a narrower error deviation and a lower coefficient variation than in a conventional 96-well plate ELISA. Furthermore, the sample requirement (1 mu L) for the hybrid-patterned chip is about 50 times less than that required in an ELISA (at least 50 mu L). The proposed hydrophobic/hydrophilic hybrid-patterned microarray chip is expected to be a highly efficient tool that can be applied to a high throughput immunoassay of a specific cancer marker.
URI
http://link.springer.com/article/10.1007%2Fs13206-012-6102-yhttp://repository.hanyang.ac.kr/handle/20.500.11754/70106
ISSN
1976-0280
DOI
10.1007/s13206-012-6102-y
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE