Expression of claudin-1 and -11 in immature and mature pheasant (Phasianus colchicus) testes
- Title
- Expression of claudin-1 and -11 in immature and mature pheasant (Phasianus colchicus) testes
- Author
- 오영석
- Keywords
- Claudins; Tight junction; Pheasant testis; FOWL GALLUS-DOMESTICUS; MOUSE SERTOLI-CELLS; TIGHT JUNCTIONS; SEMINIFEROUS EPITHELIUM; GERM-CELLS; BARRIER; RAT; PROLIFERATION; ANDROGENS; RECEPTOR
- Issue Date
- 2011-02
- Publisher
- ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
- Citation
- THERIOGENOLOGY; FEB 2011, 75 3, p445-p458, 14p.
- Abstract
- The expression of claudin-1 and -11, tight junctions (TJs) proteins was examined in immature and adult pheasant (Phasianus colchicus) testes. Claudin-1 and -11 cDNA were highly similar to those of human, mice, and chicken. Claudin-1 mRNA and protein (21 kDa) levels in immature testes were higher than those of adult testis. In immature testes until 6 weeks of age, Claudin-1 was found at contacts between adjacent Sertoli cells and between Sertoli cells and germ cells. In adult testis, Claudin-1 was found in early spermatocytes migrating the blood testis barrier (BTB). Blood vessels were positive for claudin-1. Claudin-11 mRNA and protein (21 kDa) increased during adulthood development of testis. In immature testis, Claudin-11 was found in apicolateral contacts between adjacent Sertoli cells, indicating its involvement in cell adhesion in immature testis. In adult testis, strong wavy Claudin-11 immunoreactivity was parallel to basal lamina at the basal part of seminiferous epithelium, indicating that Claudin-11 at the inter-Sertoli TJs may act as a structural element of the BTB. Weak Claudin-1 and -11 immunoreactivity at contacts between Sertoli cells to elongating/elongated spermatids, meiotic germ cells, and basal lamina suggests that they also participate in the cell cell and cell extracellular matrix adhesion in pheasant testis. Testosterone increased claudin-11 mRNA in testis organ culture and Sertoli cell primary culture, suggesting positive regulation of claudin-11 gene by androgen in Sertoli cells of pheasant testis. This is the first report on the claudins expression at BTB in avian testis. (C) 2011 Elsevier Inc. All rights reserved.
- URI
- http://www.sciencedirect.com/science/article/pii/S0093691X10004954?_rdoc=1&_fmt=high&_origin=gateway&_docanchor=&md5=b8429449ccfc9c30159a5f9aeaa92ffbhttps://repository.hanyang.ac.kr/handle/20.500.11754/69989
- ISSN
- 0093-691X
- DOI
- 10.1016/j.theriogenology.2010.09.012
- Appears in Collections:
- RESEARCH INSTITUTE[S](부설연구소) > THE RESEARCH INSTITUTE FOR NATURAL SCIENCES(자연과학연구소) > Articles
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