Expression of claudin-1 and -11 in immature and mature pheasant (Phasianus colchicus) testes

Title
Expression of claudin-1 and -11 in immature and mature pheasant (Phasianus colchicus) testes
Authors
오영석
Keywords
Claudins; Tight junction; Pheasant testis; FOWL GALLUS-DOMESTICUS; MOUSE SERTOLI-CELLS; TIGHT JUNCTIONS; SEMINIFEROUS EPITHELIUM; GERM-CELLS; BARRIER; RAT; PROLIFERATION; ANDROGENS; RECEPTOR
Issue Date
2011-02
Publisher
ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
Citation
THERIOGENOLOGY; FEB 2011, 75 3, p445-p458, 14p.
Abstract
The expression of claudin-1 and -11, tight junctions (TJs) proteins was examined in immature and adult pheasant (Phasianus colchicus) testes. Claudin-1 and -11 cDNA were highly similar to those of human, mice, and chicken. Claudin-1 mRNA and protein (21 kDa) levels in immature testes were higher than those of adult testis. In immature testes until 6 weeks of age, Claudin-1 was found at contacts between adjacent Sertoli cells and between Sertoli cells and germ cells. In adult testis, Claudin-1 was found in early spermatocytes migrating the blood testis barrier (BTB). Blood vessels were positive for claudin-1. Claudin-11 mRNA and protein (21 kDa) increased during adulthood development of testis. In immature testis, Claudin-11 was found in apicolateral contacts between adjacent Sertoli cells, indicating its involvement in cell adhesion in immature testis. In adult testis, strong wavy Claudin-11 immunoreactivity was parallel to basal lamina at the basal part of seminiferous epithelium, indicating that Claudin-11 at the inter-Sertoli TJs may act as a structural element of the BTB. Weak Claudin-1 and -11 immunoreactivity at contacts between Sertoli cells to elongating/elongated spermatids, meiotic germ cells, and basal lamina suggests that they also participate in the cell cell and cell extracellular matrix adhesion in pheasant testis. Testosterone increased claudin-11 mRNA in testis organ culture and Sertoli cell primary culture, suggesting positive regulation of claudin-11 gene by androgen in Sertoli cells of pheasant testis. This is the first report on the claudins expression at BTB in avian testis. (C) 2011 Elsevier Inc. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0093691X10004954?_rdoc=1&_fmt=high&_origin=gateway&_docanchor=&md5=b8429449ccfc9c30159a5f9aeaa92ffbhttp://repository.hanyang.ac.kr/handle/20.500.11754/69989
ISSN
0093-691X
DOI
http://dx.doi.org/10.1016/j.theriogenology.2010.09.012
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RESEARCH INSTITUTE[S](부설연구소) > THE RESEARCH INSTITUTE FOR NATURAL SCIENCES(자연과학연구소) > Articles
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