Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 진언선 | - |
dc.date.accessioned | 2018-04-19T22:27:02Z | - |
dc.date.available | 2018-04-19T22:27:02Z | - |
dc.date.issued | 2013-03 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v. 41, Page. 752-757 | en_US |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0956566312006586?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/69969 | - |
dc.description.abstract | We report the novel activity-based detection of antifreeze protein (AFP), also known as ice-binding protein (IBP), using freeze-labile gold nanoparticles (AuNPs) in order to overcome labor-intensive and low throughput issues of the current method based on thermal hysteresis (TH). Upon the addition of either CnAFP from the Antarctic diatom Chaetoceros neogracile or LeIBP from the Arctic yeast Leucosporidium sp. to mercaptosuccinic acid-capped AuNP, the self-assembly of AuNPs was highly inhibited after a freezing/thawing cycle, leading to no color change in the AuNP solution. As a result, the aggregation parameter (E520/E650) of AuNP presented the rapid detection of both the concentration-dependent activity and stability of two AFPs with high sensitivity, where the detection range was 100-fold lower than that of the TH-based method. We suggest that our newly developed method is very suitable for simple and high-throughput measurement of AFP activity. | en_US |
dc.description.sponsorship | This research was supported by the 2011 Polar Academic Program (PAP,PD11010) from KOPRI, and Basic Science Research Program (2012-0008222), the Bio-Signal Analysis Technology Innovation Pro-gram (2012-0006053) and Nano Material Technology Development Program(2012035286) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology(MEST). This work was also supported by the Korea CCS R&D Center (KCRC) grant funded by the Korea government (MEST) (2011-0031999). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | en_US |
dc.subject | Antifreeze protein | en_US |
dc.subject | Frozen assembly | en_US |
dc.subject | Gold nanoparticle | en_US |
dc.subject | Ice-binding protein | en_US |
dc.subject | Thermal hysteresis | en_US |
dc.title | Frozen assembly of gold nanoparticles for rapid analysis of antifreeze protein activity | en_US |
dc.type | Article | en_US |
dc.relation.volume | 41 | - |
dc.identifier.doi | 10.1016/j.bios.2012.09.052 | - |
dc.relation.page | 752-757 | - |
dc.relation.journal | BIOSENSORS & BIOELECTRONICS | - |
dc.contributor.googleauthor | Kim, Young-Pil | - |
dc.contributor.googleauthor | Park, Ji-In | - |
dc.contributor.googleauthor | Gwak, Yunho Yunho | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.contributor.googleauthor | Kim, Young-Pil | - |
dc.contributor.googleauthor | Lee, Jun Hyuck | - |
dc.contributor.googleauthor | Kim, Hak Jun | - |
dc.relation.code | 2009201344 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | esjin | - |
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