Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer

Title
Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer
Author
김영필
Keywords
protease; quantum dot; energy transfer; BRET; FRET; multiplex; nanoparticle; luciferase; quencher
Issue Date
2012-02
Publisher
Ivyspring International Publisher
Citation
Theranostics, 2012, 2(2), P.127~165
Abstract
This review demonstrates the detection of protease activity based on the energy transfer of quantum dots (QDs). By incorporation of varying protease substrates into designed QD probes both in fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) system, proteolytic activity led to changes in the energy transfer efficiency. Especially due to the superior properties of QDs, it can be served as an excellent probe for a multiplexed and high-throughput protease assay with high sensitivity. It is anticipated that the QD-based FRET/BRET probes will have a great potential for dissecting the fundamental roles of proteases and designing potential protease inhibitors as therapeutic drugs in biology and nanomedicine.
URI
http://www.thno.org/v02p0127.htmhttp://repository.hanyang.ac.kr/handle/20.500.11754/69945
ISSN
1838-7640
DOI
10.7150/thno.3476
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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