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Heat Shock Pretreatment Reduces Intestinal Injury in a Neonatal Rat Model of Early Necrotizing Enterocolitis

Title
Heat Shock Pretreatment Reduces Intestinal Injury in a Neonatal Rat Model of Early Necrotizing Enterocolitis
Author
이현주
Keywords
Necrotizing enterocolitis; Heat shock protein 70; Nuclear factor-kappa B
Issue Date
2013-01
Publisher
S. Karger AG
Citation
NEONATOLOGY, 2012, 103(1), P.1-6
Abstract
Background: Increased pro-inflammatory cytokines are suggested in the pathogenesis of necrotizing enterocolitis (NEC). The transcription factor, nuclear factor-kappa B (NF-kappa B), is a central regulator of inflammatory and immune responses, and recent rodent NEC models have shown that NF-kappa B may have a critical role in the disease processes that underlie NEC. Heat shock proteins have important functions in response to stress-related events in a variety of systems, including digestive organs. Objectives: We investigated whether heat shock pretreatment protects intestinal epithelial damage in the early NEC rat model. Methods: We generated human NEC-like lesions in neonatal rat ileum by administering oral endotoxin (10 mg/kg), intermittent 8% hypoxia, and hypertonic formula. Heat shock was administered by raising the chamber temperature to 42 degrees C for 20 min, 3 h prior to endotoxin ingestion. Results: Heat shock pretreatment increased the expression of HSP70 in the ileal tissue and attenuated histological severity of early experimental NEC. NF-kappa B was activated in the ileal tissue of the NEC group and this activation was attenuated by heat shock pretreatment, which was determined by electrophoretic mobility shift assay and Western blot analysis of p50 in subcellular fractionated samples. Conclusions: Heat shock pretreatment reduced the incidence and severity of early experimental NEC in rats. A possible mechanism underlying this protective effect includes inhibition of NF-kappa B activation through increased HSP70 expression. Copyright (C) 2012 S. Karger AG, Basel
URI
https://www.karger.com/Article/Abstract/339179https://repository.hanyang.ac.kr/handle/20.500.11754/69825
ISSN
1661-7800
DOI
10.1159/000339179
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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