93 0

Detection of Single Nucleotide Polymorphisms Using a Biosensor-Containing Titanium-Well Array

Title
Detection of Single Nucleotide Polymorphisms Using a Biosensor-Containing Titanium-Well Array
Author
박진구
Keywords
SNP Detection; DNA Sensor; Anti-Biofouling Array Sensor; Titanium Microarray; Amine-Modified Microarray; Fluorescence Scanner; DNA; GENOME
Issue Date
2013-01
Publisher
American Scientific Publishers
Citation
Journal of Nanoscience and Nanotechnology, 2013, 13(1), P.139-143
Abstract
The rapid identification and verification of single nucleotide polymorphisms (SNPs) were demonstrated using a well array sensor containing anti-biofouling titanium (Ti). Probe single-stranded DNA (ssDNA) was immobilized inside a titanium-well array on amine-modified glass surfaces with anti-biofouling behavior via a streptavidin-biotin interaction. Fluorescence intensity changes originating from the hybridization of nucleic acids to protein-bound nucleic acids linked to Alexa Fluor (FL) 647 were observed. The protocol was highly sensitive and reproducible for the detection of DNA hybridization. Significant changes in fluorescence signals were observed when using target DNA with a single base mismatch, indicating that this method is applicable to SNP detection. The microarray technology for the detection of SNPs using anti-biofouling Ti and other methods can be used as a highly sensitive in vitro medical sensor, as highlighted by an increase in genotyping accuracy.
URI
http://www.ingentaconnect.com/content/asp/jnn/2013/00000013/00000001/art00016?crawler=truehttp://repository.hanyang.ac.kr/handle/20.500.11754/69741
ISSN
1533-4880
DOI
10.1166/jnn.2013.6865
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE