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dc.contributor.author김진웅-
dc.date.accessioned2018-04-19T08:49:13Z-
dc.date.available2018-04-19T08:49:13Z-
dc.date.issued2012-03-
dc.identifier.citationApplied Biochemistry and Biotechnology, 2012, 166(5), P.1137-1147en_US
dc.identifier.issn0273-2289-
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs12010-011-9500-z-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/69562-
dc.description.abstractAmentoflavone is a well-known biflavonoid that has diverse biological effects. Previously, we reported that amentoflavone suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression in normal human fibroblasts (NHF). We investigated the effects of amentoflavone on UVB-induced MMP-1 expression in order to elucidate its mode of action. NHF were treated with amentoflavone for indicated times and doses with UVB irradiation. The expressions of MMP-1 gene and protein were determined by RT-PCR and ELISA, respectively. MAP kinase phosphorylation and the expression of c-Fos protein were determined by Western blot. The treatment of amentoflavone completely blocked the upregulation of MMP-1 which is induced by UVB irradiation in HaCaT-NHF co-culture in a dose-dependent manner as well as in NHF monoculture. Also, amentoflavone inhibited UVB-induced activation of extracellular signal-regulated kinase (ERK) without changing total ERK protein level, and did not affect p38 or JNK activation. Finally, AP-1 transcription factor components, phospho-c-Jun and c-Fos protein expressions were decreased by amentoflavone treatment. The major finding of this study shows that amentoflavone inhibits intracellular cell signaling ERK pathway leading to the prevention of MMP-1 expression in human skin fibroblasts. Therefore, these results strongly suggest that amentoflavone should be investigated as a potential agent for the prevention and the treatment of skin photoaging.en_US
dc.description.sponsorshipThe study was financially supported by the research fund of Hanyang University (HY-2011-N).en_US
dc.language.isoenen_US
dc.publisherHUMANA PRESS INCen_US
dc.subjectAmentoflavoneen_US
dc.subjectUltraviolet-Ben_US
dc.subjectMatrix metalloproteinase-1en_US
dc.subjectMAP kinaseen_US
dc.subjectAP-1en_US
dc.subjectHuman fibroblastsen_US
dc.titleAmentoflavone Inhibits UVB-Induced Matrix Metalloproteinase-1 Expression Through the Modulation of AP-1 Components in Normal Human Fibroblastsen_US
dc.typeArticleen_US
dc.relation.no5-
dc.relation.volume166-
dc.identifier.doi10.1007/s12010-011-9500-z-
dc.relation.page1137-1147-
dc.relation.journalAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY-
dc.contributor.googleauthorLee, C. W.-
dc.contributor.googleauthorNa, Y.-
dc.contributor.googleauthorPark, N. h.-
dc.contributor.googleauthorKim, H. S.-
dc.contributor.googleauthorAhn, S. M.-
dc.contributor.googleauthorKim, J. W.-
dc.contributor.googleauthorKim, H. K.-
dc.contributor.googleauthorJang, Y. P.-
dc.relation.code2012200828-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidkjwoong-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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