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dc.contributor.author신인철-
dc.date.accessioned2018-04-19T08:46:55Z-
dc.date.available2018-04-19T08:46:55Z-
dc.date.issued2012-03-
dc.identifier.citationInternational Journal of Oncology, 2012, 40(3), P.665-672en_US
dc.identifier.issn1019-6439-
dc.identifier.urihttps://www.spandidos-publications.com/10.3892/ijo.2011.1289-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/69559-
dc.description.abstractPrimary breast sarcomas (PBSs) that arise from mammary stroma are very rare, highly aggressive and therapy-resistant tumors with a heterogeneous phenotype. In this study, we sought to identify tumor-initiating cells (TICs) in PBSs and to describe their features. We isolated long-term self-renewing sarcospheres (designated NDY-1) from primary breast carcinosarcoma tissue (sarcoma component >95%) using the anchorage-independent culture method. NDY-1 spheres expressed various mesenchymal cell markers, and their tumorigenic potential was markedly reduced in adherent culture conditions, compared to spheres. Screening for integrins revealed a marked decrease in CD49d expression in adherent culture conditions of NDY-1. The CD49d(+/high) subpopulation sorted from NDY-1 spheres displayed higher cell viability and sphere-forming ability than CD49d(-/low) population in vitro. Moreover, the CD49d(+/high) population displayed high tumor initiating ability in limiting dilution transplantation to NOD/SCID mice, and the xenotransplanted CD49d(+/high) population recapitulated the complexity of the original primary tumors. Greater doxorubicin resistance was exhibited by the CD49d(+/high) population, compared with the CD49d(-/low) population. Thus, our results collectively demonstrate that CD49d(+/high) cells from sarcospheres display enhanced sphere-forming, drug resistance and tumor-initiating abilities. To our knowledge, this is the first study to identify TICs from breast sarcoma.en_US
dc.description.sponsorshipThis study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A084022) and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0004148).en_US
dc.language.isoenen_US
dc.publisherSPANDIDOS PUBL LTDen_US
dc.subjectBreast sarcomaen_US
dc.subjectCD49den_US
dc.subjectTumor-initiating cellsen_US
dc.titleThe CD49d(+/high) subpopulation from isolated human breast sarcoma spheres possesses tumor-initiating abilityen_US
dc.title.alternativehigh) subpopulation from isolated human breast sarcoma spheres possesses tumor-initiating abilityen_US
dc.typeArticleen_US
dc.relation.no3-
dc.relation.volume40-
dc.identifier.doi10.3892/ijo.2011.1289-
dc.relation.page665-672-
dc.relation.journalINTERNATIONAL JOURNAL OF ONCOLOGY-
dc.contributor.googleauthorLee, K.-M.-
dc.contributor.googleauthorHan, W.1,-
dc.contributor.googleauthorKim, J.B.-
dc.contributor.googleauthorKo, E.-
dc.contributor.googleauthorNoh, D.-Y.-
dc.contributor.googleauthorShin, I.-
dc.contributor.googleauthorPark, I.A.-
dc.contributor.googleauthorLee, D.S.-
dc.contributor.googleauthorOh, K.-
dc.relation.code2012204282-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidincheol-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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