Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신인철 | - |
dc.date.accessioned | 2018-04-19T08:46:55Z | - |
dc.date.available | 2018-04-19T08:46:55Z | - |
dc.date.issued | 2012-03 | - |
dc.identifier.citation | International Journal of Oncology, 2012, 40(3), P.665-672 | en_US |
dc.identifier.issn | 1019-6439 | - |
dc.identifier.uri | https://www.spandidos-publications.com/10.3892/ijo.2011.1289 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/69559 | - |
dc.description.abstract | Primary breast sarcomas (PBSs) that arise from mammary stroma are very rare, highly aggressive and therapy-resistant tumors with a heterogeneous phenotype. In this study, we sought to identify tumor-initiating cells (TICs) in PBSs and to describe their features. We isolated long-term self-renewing sarcospheres (designated NDY-1) from primary breast carcinosarcoma tissue (sarcoma component >95%) using the anchorage-independent culture method. NDY-1 spheres expressed various mesenchymal cell markers, and their tumorigenic potential was markedly reduced in adherent culture conditions, compared to spheres. Screening for integrins revealed a marked decrease in CD49d expression in adherent culture conditions of NDY-1. The CD49d(+/high) subpopulation sorted from NDY-1 spheres displayed higher cell viability and sphere-forming ability than CD49d(-/low) population in vitro. Moreover, the CD49d(+/high) population displayed high tumor initiating ability in limiting dilution transplantation to NOD/SCID mice, and the xenotransplanted CD49d(+/high) population recapitulated the complexity of the original primary tumors. Greater doxorubicin resistance was exhibited by the CD49d(+/high) population, compared with the CD49d(-/low) population. Thus, our results collectively demonstrate that CD49d(+/high) cells from sarcospheres display enhanced sphere-forming, drug resistance and tumor-initiating abilities. To our knowledge, this is the first study to identify TICs from breast sarcoma. | en_US |
dc.description.sponsorship | This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A084022) and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0004148). | en_US |
dc.language.iso | en | en_US |
dc.publisher | SPANDIDOS PUBL LTD | en_US |
dc.subject | Breast sarcoma | en_US |
dc.subject | CD49d | en_US |
dc.subject | Tumor-initiating cells | en_US |
dc.title | The CD49d(+/high) subpopulation from isolated human breast sarcoma spheres possesses tumor-initiating ability | en_US |
dc.title.alternative | high) subpopulation from isolated human breast sarcoma spheres possesses tumor-initiating ability | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 40 | - |
dc.identifier.doi | 10.3892/ijo.2011.1289 | - |
dc.relation.page | 665-672 | - |
dc.relation.journal | INTERNATIONAL JOURNAL OF ONCOLOGY | - |
dc.contributor.googleauthor | Lee, K.-M. | - |
dc.contributor.googleauthor | Han, W.1, | - |
dc.contributor.googleauthor | Kim, J.B. | - |
dc.contributor.googleauthor | Ko, E. | - |
dc.contributor.googleauthor | Noh, D.-Y. | - |
dc.contributor.googleauthor | Shin, I. | - |
dc.contributor.googleauthor | Park, I.A. | - |
dc.contributor.googleauthor | Lee, D.S. | - |
dc.contributor.googleauthor | Oh, K. | - |
dc.relation.code | 2012204282 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | incheol | - |
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