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The CD49d(+/high) subpopulation from isolated human breast sarcoma spheres possesses tumor-initiating ability

Title
The CD49d(+/high) subpopulation from isolated human breast sarcoma spheres possesses tumor-initiating ability
Other Titles
high) subpopulation from isolated human breast sarcoma spheres possesses tumor-initiating ability
Author
신인철
Keywords
Breast sarcoma; CD49d; Tumor-initiating cells
Issue Date
2012-03
Publisher
SPANDIDOS PUBL LTD
Citation
International Journal of Oncology, 2012, 40(3), P.665-672
Abstract
Primary breast sarcomas (PBSs) that arise from mammary stroma are very rare, highly aggressive and therapy-resistant tumors with a heterogeneous phenotype. In this study, we sought to identify tumor-initiating cells (TICs) in PBSs and to describe their features. We isolated long-term self-renewing sarcospheres (designated NDY-1) from primary breast carcinosarcoma tissue (sarcoma component >95%) using the anchorage-independent culture method. NDY-1 spheres expressed various mesenchymal cell markers, and their tumorigenic potential was markedly reduced in adherent culture conditions, compared to spheres. Screening for integrins revealed a marked decrease in CD49d expression in adherent culture conditions of NDY-1. The CD49d(+/high) subpopulation sorted from NDY-1 spheres displayed higher cell viability and sphere-forming ability than CD49d(-/low) population in vitro. Moreover, the CD49d(+/high) population displayed high tumor initiating ability in limiting dilution transplantation to NOD/SCID mice, and the xenotransplanted CD49d(+/high) population recapitulated the complexity of the original primary tumors. Greater doxorubicin resistance was exhibited by the CD49d(+/high) population, compared with the CD49d(-/low) population. Thus, our results collectively demonstrate that CD49d(+/high) cells from sarcospheres display enhanced sphere-forming, drug resistance and tumor-initiating abilities. To our knowledge, this is the first study to identify TICs from breast sarcoma.
URI
https://www.spandidos-publications.com/10.3892/ijo.2011.1289http://repository.hanyang.ac.kr/handle/20.500.11754/69559
ISSN
1019-6439
DOI
10.3892/ijo.2011.1289
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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