3,4-Dihydroxyphenylalanine-Assisted Hydroxyapatite Nanoparticle Coating on Polymer Scaffolds for Efficient Osteoconduction

Title
3,4-Dihydroxyphenylalanine-Assisted Hydroxyapatite Nanoparticle Coating on Polymer Scaffolds for Efficient Osteoconduction
Author
이민형
Keywords
BONELIKE APATITE GROWTH; COMPOSITE SCAFFOLDS; IN-VITRO; TISSUE; MICROSPHERES; BIOMATERIALS; REPLACEMENT; MEMBRANES; ADHESION; BEHAVIOR
Issue Date
2012-03
Publisher
Mary Ann Leibert INC
Citation
Tissue Engineering C, 2012, 18(4), P.245-251
Abstract
For bone regeneration applications, scaffolds made from a composite of a biodegradable polymer and ceramic have advantages over scaffolds made from only one component (biodegradable polymer or ceramic alone). In this study, a simple and rapid method was developed to induce hydroxyapatite (HA) nanoparticle adsorption on polyglycolic acid (PGA) scaffold surfaces. PGA meshes were coated with HA nanoparticles by immersing the scaffolds in a buffer solution containing 3,4-dihydroxyphenylalanine (DOPA), a critical, functional element in mussel adhesive protein known to strongly bind to various materials. Substantial HA coating on PGA scaffolds was achieved within 24 hours of immersion, as determined according to selective staining of ceramic particles, scanning electron microscopy, X-ray photoelectron spectroscopy, and energy-dispersive spectroscopy. To evaluate the osteoconduction efficacy of the scaffolds in vivo, PGA scaffolds, DOPA-coated PGA scaffolds, PGA scaffolds immersed in HA solution, and HA- and DOPA-coated PGA (HA-DOPA-PGA) scaffolds were implanted in critical-sized defects in mouse skulls for 8 weeks. Micro-computed tomography and histological analyses showed that bone regeneration in vivo was far more extensive on HA-DOPA-PGA scaffolds than on the other scaffolds. DOPA offers an efficient and simple method of HA coating on polymer scaffolds. HA- polymer composite scaffolds fabricated using this method could be useful as bone graft.
URI
https://www.liebertpub.com/doi/abs/10.1089/ten.tec.2011.0373http://repository.hanyang.ac.kr/handle/20.500.11754/69498
ISSN
1937-3384; 1937-3392
DOI
10.1089/ten.tec.2011.0373
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE