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Coenzyme Q10 protects against amyloid beta-induced neuronal cell death by inhibiting oxidative stress and activating the P13K pathway

Title
Coenzyme Q10 protects against amyloid beta-induced neuronal cell death by inhibiting oxidative stress and activating the P13K pathway
Author
이규용
Keywords
Amyloid-beta; Coenzyme Q10; Oxidative stress; Neuroprotection; Phosphatidylinositol 3-kinase
Issue Date
2012-01
Publisher
Elsevier Science B.V., Amsterdam.
Citation
Neurotoxicology, January 2012, 33(1), p.85-90
Abstract
Oxidative stress plays critical roles in the pathogenic mechanisms of several neurodegenerative disorders including Alzheimer's disease (AD), thus much research effort has focused on antioxidants as potential treatment agents for AD. Coenzyme Q10 (CoQ10) is known to have powerful antioxidant effects. We investigated the neuroprotective effects of CoQ10 against Amyloid beta(25-35) (A beta(25-35))-induced neurotoxicity in rat cortical neurons. To evaluate the neuroprotective effects of CoQ10 on A beta(25-35)-injured neurons, primary cultured cortical neurons were treated with several concentrations of CoQ10 and/or A beta(25-35) for 48 h. CoQ10 protected neuronal cells against A beta(25-35)-induced neurotoxicity in a concentration-dependent manner. These neuroprotective effects of CoQ10 were blocked by LY294002 (10 mu M), a phosphatidylinositol 3-kinase (P13K) inhibitor. A beta(25-35) concentration-dependent increased free radical levels in rat cortical neurons, while combined treatment with CoQ10 reduced these free radical levels in a dose-dependent manner. Meanwhile. CoQ10 treatment of A beta(25-35)-injured primary cultured cortical neurons increased the expression levels of p85aP13K, phosphorylated Akt, phosphorylated glycogen synthase kinase-3 beta, and heat shock transcription factor, which are proteins related to neuronal cell survival, and decreased the levels of cytosolic cytochrome c and cleaved caspase-3, which are associated with neuronal cell death. Together, these results suggest that the neuroprotective effects of CoQ10 on A beta(25-35) neurotoxicity are mediated by inhibition of oxidative stress together with activation of the P13-K/Akt pathway. (C) 2011 Elsevier Inc. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0161813X11002142http://repository.hanyang.ac.kr/handle/20.500.11754/69353
ISSN
0161-813X
DOI
10.1016/j.neuro.2011.12.005
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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