Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 유혜현 | - |
dc.date.accessioned | 2018-04-19T04:51:57Z | - |
dc.date.available | 2018-04-19T04:51:57Z | - |
dc.date.issued | 2016-09 | - |
dc.identifier.citation | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v. 129, Page. 90-95 | en_US |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.issn | 1873-264X | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0731708516303569 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/69333 | - |
dc.description.abstract | Hederacoside C is a principal bioactive pharmaceutical ingredient of Hedera helix leaf extracts. H. helix extracts have long been used in folk medicine for the treatment of respiratory disorders. Currently, hederacoside C is investigated as a promising candidate for the treatment of respiratory diseases. In this study, an accurate, sensitive, rapid, and reliable bioanalytical method was developed for the determination of hederacoside C in rat plasma using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). For sample preparation, plasma proteins were precipitated with 0.1% acetic acid in acetonitrile. Waters UPLC BEH C-18 (2.1 mm I.D. x 100 mm, 1.7 mu m) column was used for chromatographic separation. A gradient elution of mobile phases consisting of 0.02% acetic acid in distilled water (solvent A) and 0.02% acetic acid in acetonitrile (solvent B) was used at a flow rate of 0.3 mL/min. The multiple reaction monitoring (MRM) mode was used for mass spectrometric detection; the MRM transitions were m/z 1219.7 -> m/z 469.2 for hederacoside C and m/z 1108.3 -> m/z 221.2 for ginsenoside Rb1 (internal standard) in the negative ionization mode. A calibration curve was constructed in the range of 10-1000 ng/mL. The intra- and inter-day precision and accuracy were within 5%. The developed UPLC-MS/MS method was successfully applied in a pharmacokinetic study of hederacoside C in rats. Hederacoside C was quickly but inadequately absorbed from the gastrointestinal tract of rats resulting in extremely low bioavailability and relatively slow clearance. (C) 2016 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry (NRF-2014R1A1A1A05002840). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Hederacoside C | en_US |
dc.subject | Hedera helix | en_US |
dc.subject | UPLC-MS/MS | en_US |
dc.subject | Plasma | en_US |
dc.subject | Pharmacokinetics | en_US |
dc.subject | ALPHA-HEDERIN | en_US |
dc.subject | HELIX | en_US |
dc.subject | SAPONINS | en_US |
dc.subject | EXTRACT | en_US |
dc.subject | LEAVES | en_US |
dc.subject | PHARMACOKINETICS | en_US |
dc.subject | HEDERAGENIN | en_US |
dc.subject | FRUITS | en_US |
dc.title | An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma | en_US |
dc.type | Article | en_US |
dc.relation.volume | 129 | - |
dc.identifier.doi | 10.1016/j.jpba.2016.06.039 | - |
dc.relation.page | 90-95 | - |
dc.relation.journal | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | - |
dc.contributor.googleauthor | Rehman, SU | - |
dc.contributor.googleauthor | Choi, MS | - |
dc.contributor.googleauthor | Kim, IS | - |
dc.contributor.googleauthor | Kim, S.H | - |
dc.contributor.googleauthor | Yoo, HH | - |
dc.relation.code | 2016000071 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | yoohh | - |
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