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An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma

Title
An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma
Author
유혜현
Keywords
Hederacoside C; Hedera helix; UPLC-MS/MS; Plasma; Pharmacokinetics; ALPHA-HEDERIN; HELIX; SAPONINS; EXTRACT; LEAVES; PHARMACOKINETICS; HEDERAGENIN; FRUITS
Issue Date
2016-09
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v. 129, Page. 90-95
Abstract
Hederacoside C is a principal bioactive pharmaceutical ingredient of Hedera helix leaf extracts. H. helix extracts have long been used in folk medicine for the treatment of respiratory disorders. Currently, hederacoside C is investigated as a promising candidate for the treatment of respiratory diseases. In this study, an accurate, sensitive, rapid, and reliable bioanalytical method was developed for the determination of hederacoside C in rat plasma using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). For sample preparation, plasma proteins were precipitated with 0.1% acetic acid in acetonitrile. Waters UPLC BEH C-18 (2.1 mm I.D. x 100 mm, 1.7 mu m) column was used for chromatographic separation. A gradient elution of mobile phases consisting of 0.02% acetic acid in distilled water (solvent A) and 0.02% acetic acid in acetonitrile (solvent B) was used at a flow rate of 0.3 mL/min. The multiple reaction monitoring (MRM) mode was used for mass spectrometric detection; the MRM transitions were m/z 1219.7 -> m/z 469.2 for hederacoside C and m/z 1108.3 -> m/z 221.2 for ginsenoside Rb1 (internal standard) in the negative ionization mode. A calibration curve was constructed in the range of 10-1000 ng/mL. The intra- and inter-day precision and accuracy were within 5%. The developed UPLC-MS/MS method was successfully applied in a pharmacokinetic study of hederacoside C in rats. Hederacoside C was quickly but inadequately absorbed from the gastrointestinal tract of rats resulting in extremely low bioavailability and relatively slow clearance. (C) 2016 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0731708516303569http://repository.hanyang.ac.kr/handle/20.500.11754/69333
ISSN
0731-7085; 1873-264X
DOI
10.1016/j.jpba.2016.06.039
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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