바이오 연료생산용 담수미세조류의 파일럿규모 재배 및 수확

Title
바이오 연료생산용 담수미세조류의 파일럿규모 재배 및 수확
Other Titles
Pilot-scale cultivation and harvesting of freshwater microalgae in various wastewaters for bioenergy production
Author
양일승
Alternative Author(s)
Yang, Il Seung
Advisor(s)
전병훈
Issue Date
2018-02
Publisher
한양대학교
Degree
Doctor
Abstract
Abstract Pilot-scale cultivation and harvesting of freshwater microalgae in various wastewaters for bioenergy production Il-Seung Yang Department of Earth Resources and Environmental Engineering, The Graduate School, Hanyang University, South Korea Microalgal biomass is considered as a 3rd generation biomass which does not need arable land for cultivation, typically has a high productivity, and can be converted to a widespread of energy carriers. Algal biomass has received a significant attention in research focused on chemical and bioenergy production. The most significant blockades for economical biofuel production using algae are the technological and engineering aspects, such as isolation, selection of potent microalgae species, and high cost of microalgae cultivation and harvesting stages. Selection of microalgae strains and optimization of the culture condition are key growth parameters to maximize biomass production and nutrient uptake from wastewater. Moreover, improvement of microalgae growth and their biochemical composition such as fatty acids are urgently required for better biodiesel production. In this research work, we have tried to enhance the microalgal growth and fatty acid production which is essential for development of a microalgae-based biodiesel production. Three different microalgal species (S. obliquus GU732418, O. multisporus GU732424 and C. vulgaris FR751187) were individually cultivated in media containing both indole-3-acetic acid (IAA) and diethyl aminoethyl hexanoate (DAH) at concentrations of 10-810-4 M. Combined phytohormones (10-8 to 10-5 M) treatment increased the growth of all three species when compared to control growth media. IAA and DAH supported the maximum growth of S. obliquus (38.12×106 cells mL-1) at 10-8 M, O. multisporus (85.89×106 cells mL-1) at 10-6 M, and C. vulgaris (4.09×106 cells mL-1) at 10-5 M. Addition of 10-7 M IAA and DAH also assisted in the removal of K+ (88%), Zn2+ (97%) and Mg2+ (99%) from the media by S. obliquus. The highest removal of K+, Zn2+, and Mg2+ by C. vulgaris was achieved at 10-5 M IAA and DAH. Under all experimental conditions (10-810-4 M IAA and DAH), the amounts of poly-unsaturated fatty acids were significantly increased. Palmitic acid, linoleic acid and γ-linolenic acid were the major fatty acids, accounting for 11.7521.55%, 2.556.73%, and 52.9375.89% of the total fatty acid content, respectively. The fatty acids that accumulated in O. multisporus and C. vulgaris were found to be suitable to produce high quality biodiesel with characteristics equivalent to crop seed oil-derived biodiesel. Microalgae, C. vulgaris and S. obliquus were cultivated in a small scale vertical flat-plate photobioreactors (PBRs) supplemented with municipal wastewater in order to achieve simultaneous wastewater treatment and biomass production for biofuel generation. Microalgal growth and nutrient removal including total nitrogen (TN), total phosphorus (TP), total inorganic carbon (TIC) and trace elements (Na+, Ca2+, Mg2+, and Zn2+) were monitored during microalgae cultivation. C. vulgaris and S. obliquus showed optimal specific growth rates (µopt) of 1.39 and 1.41 day-1, respectively, and the TN and TP were completely removed (>99%) from the wastewater within 8 days. Microalgal biomass in the PBR was harvested using a natural flocculant produced from M. oleifera seeds. The harvesting efficiency of M. oleifera was 81% for C. vulgaris and 92% for S. obliquus. The amounts of saturated, mono-unsaturated, and poly-unsaturated fatty acids in the harvested biomass accounted for 18.66, 71.61 and 9.75% for C. vulgaris, and 28.67, 57.14 and 11.15% for S. obliquus, respectively. The accumulated fatty acids were suitable to produce high quality biodiesel with characteristics equivalent to crop seeds oil- derived biodiesel. This study demonstrates the potential of microalgae-based biodiesel production through the coupling of advanced wastewater treatment with microalgae cultivation for low-cost biomass production in a PBRs.
URI
http://www.dcollection.net/handler/hanyang/000000104822http://repository.hanyang.ac.kr/handle/20.500.11754/68459
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > EARTH RESOURCES AND ENVIRONMENTAL ENGINEERING(자원환경공학과) > Theses (Ph.D.)
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