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그래핀 옥사이드와 금 나노입자를 이용한 나노자임 제조와 전기화학 바이오센서로의 응용

Title
그래핀 옥사이드와 금 나노입자를 이용한 나노자임 제조와 전기화학 바이오센서로의 응용
Other Titles
Synthesis of Nanozymes Using Graphene Oxide and Gold Nanoparticle and Its Application to Electrochemical Biosensor
Author
진가현
Advisor(s)
성기훈
Issue Date
2018-02
Publisher
한양대학교
Degree
Master
Abstract
In this study, we fabricated a nafion/graphene oxide-gold nanoparticle (GO-AuNP) hybrid modified ITO electrode and proposed an electrochemical method to detect hydrogen peroxide (H2O2) using 3,3,5,5,-tetramethylbenzidine (TMB) as a redox mediator. The GO-AuNP hybrids were prepared with a simple procedure and characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). The resulting composites were employed as nanozymes, which function as peroxidase mimics and show highly effective catalytic activity. It has been suggested that the improved catalytic activity can be attributed to the promoted electron transfer between nanozymes and substrates and the increased initial adsorption of substrates. Based on the high catalytic activity, enzyme mimics were entrapped on the ITO electrode to construct an electrochemical H2O2 sensor by casting nafion film as a secondary layer. During the catalytic reaction, the peroxidase substrate TMB was oxidized to form the TMB oxidation product, which not only produces a blue color detected by absorbance change, but also generates an electrochemical current. As a result, both spectrophotometric and electrochemical methods were used to determine H2O2 concentration. The spectrophotometric detection displayed a linearity for H2O2 concentration from 10 μM to 5 mM (r2=0.989), with an estimated detection limit of 2.0 μM. In the electrochemical detection, the TMB peak current had a good linear relationship with H2O2 concentration from 10 nM to 10 mM, with an estimated detection limit of 1.9 nM, which was much lower than that of the spectrophotometric method result. Furthermore, the electrochemical detection method also exhibited good sensitivity, reproducibility, and reliability.
URI
http://www.dcollection.net/handler/hanyang/000000105569http://repository.hanyang.ac.kr/handle/20.500.11754/68102
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Theses (Master)
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