Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이영복 | - |
dc.date.accessioned | 2018-04-18T05:50:59Z | - |
dc.date.available | 2018-04-18T05:50:59Z | - |
dc.date.issued | 2016-05 | - |
dc.identifier.citation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v. 37, NO 6, Page. 810-814 | en_US |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.issn | 1229-5949 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.1002/bkcs.10773 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/68086 | - |
dc.description.abstract | Affording several orders of signal enhancement compared to conventional nuclear magnetic resonance (NMR), dynamic nuclear polarization (DNP) enables direct observation of the most common C-13 nuclei in organic chemistry and biochemistry at low concentrations. These nuclei have poor sensitivity due to low natural abundance and a low gyromagnetic ratio. Herein, we report an application of this technique to a multi-step enzymatic reaction. The main aim of this study was to demontrate the effect of deuteration on specific spins of interest for the real-time trypsin hydrolysis reaction of synthesized N-alpha-benzoyl-L-arginine methyl-d(3) ester (BAME-d(3)). Kinetic parameter and fit accuracy were analyzed in order to determine the influence of deuteration. | en_US |
dc.description.sponsorship | This work was supported by the research fund of Hanyang University (HY-2014-N). | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-V C H VERLAG GMBH | en_US |
dc.subject | Hyperpolarization | en_US |
dc.subject | Nuclear magnetic resonance spectroscopy | en_US |
dc.subject | Enzyme kinetics | en_US |
dc.subject | Deuteration | en_US |
dc.subject | Reaction intermediates | en_US |
dc.title | Hyperpolarized NMR Analysis of Enzymatic Reaction: Extension of Observable Reaction Time by Deuterium Isotope Labeling | en_US |
dc.type | Article | en_US |
dc.relation.no | 6 | - |
dc.relation.volume | 37 | - |
dc.identifier.doi | 10.1002/bkcs.10773 | - |
dc.relation.page | 810-814 | - |
dc.relation.journal | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.contributor.googleauthor | Lee, Youngbok | - |
dc.relation.code | 2016002474 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | yblee | - |
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