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Hyperpolarized NMR Analysis of Enzymatic Reaction: Extension of Observable Reaction Time by Deuterium Isotope Labeling

Title
Hyperpolarized NMR Analysis of Enzymatic Reaction: Extension of Observable Reaction Time by Deuterium Isotope Labeling
Author
이영복
Keywords
Hyperpolarization; Nuclear magnetic resonance spectroscopy; Enzyme kinetics; Deuteration; Reaction intermediates
Issue Date
2016-05
Publisher
WILEY-V C H VERLAG GMBH
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v. 37, NO 6, Page. 810-814
Abstract
Affording several orders of signal enhancement compared to conventional nuclear magnetic resonance (NMR), dynamic nuclear polarization (DNP) enables direct observation of the most common C-13 nuclei in organic chemistry and biochemistry at low concentrations. These nuclei have poor sensitivity due to low natural abundance and a low gyromagnetic ratio. Herein, we report an application of this technique to a multi-step enzymatic reaction. The main aim of this study was to demontrate the effect of deuteration on specific spins of interest for the real-time trypsin hydrolysis reaction of synthesized N-alpha-benzoyl-L-arginine methyl-d(3) ester (BAME-d(3)). Kinetic parameter and fit accuracy were analyzed in order to determine the influence of deuteration.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/bkcs.10773http://repository.hanyang.ac.kr/handle/20.500.11754/68086
ISSN
0253-2964; 1229-5949
DOI
10.1002/bkcs.10773
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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