Epsin1 modulates synaptic vesicle retrieval capacity at CNS synapses

Title
Epsin1 modulates synaptic vesicle retrieval capacity at CNS synapses
Author
김대환
Keywords
CLATHRIN-MEDIATED ENDOCYTOSIS; HIPPOCAMPAL SYNAPSES; ENTH DOMAIN; NERVE-TERMINALS; PROTEIN; DROSOPHILA; UBIQUITINATION; PHOSPHATASE; EXOCYTOSIS; CURVATURE
Issue Date
2016-08
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v. 6, Article no. 31997
Abstract
Synaptic vesicle retrieval is an essential process for continuous maintenance of neural information flow after synaptic transmission. Epsin1, originally identified as an EPS15-interacting protein, is a major component of clathrin-mediated endocytosis. However, the role of Epsin1 in synaptic vesicle endocytosis at CNS synapses remains elusive. Here, we showed significantly altered synaptic vesicle endocytosis in neurons transfected with shRNA targeting Epsin1 during/after neural activity. Endocytosis was effectively restored by introducing shRNA-insensitive Epsin1 into Epsin1-depleted neurons. Domain studies performed on neurons in which domain deletion mutants of Epsin1 were introduced after Epsin1 knockdown revealed that ENTH, CLAP, and NPFs are essential for synaptic vesicle endocytosis, whereas UIMs are not. Strikingly, the efficacy of the rate of synaptic vesicle retrieval ( the "endocytic capacity") was significantly decreased in the absence of Epsin1. Thus, Epsin1 is required for proper synaptic vesicle retrieval and modulates the endocytic capacity of synaptic vesicles.
URI
https://www.nature.com/articles/srep31997http://hdl.handle.net/20.500.11754/68029
ISSN
2045-2322
DOI
10.1038/srep31997
Appears in Collections:
RESEARCH INSTITUTE[E](부설연구소) > INSTITUTE OF PHARMACEUTICAL SCIENCE AND TECHNOLOGY(약학기술연구소) > Articles
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