Lipopolysaccharide-mediated protein expression profiling on neuronal differentiated SH-SY5Y cells

Title
Lipopolysaccharide-mediated protein expression profiling on neuronal differentiated SH-SY5Y cells
Author
채영규
Keywords
SH-SY5Y cells; Lipopolysaccharide; MetaCore pathway analysis software (GeneGo); Neuroinflammation
Issue Date
2012-06
Publisher
Korean Biochip Society
Citation
Biochip journal, 2012, 6(2), P.165-173
Abstract
Neuroinflammation can contribute to neuronal dysfunction, death and several neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and multiple sclerosis. Lipopolysaccharide (LPS)-induced neuroinflammation severely affects neurons and can contribute to neuronal dysfunction and degeneration by causing the release of inflammatory and neurotoxic factors. We evaluated the long-term effects of treating differentiated SH-SY5Y cells with LPS to mimic LPS-induced neuroinflammation. Using matrix assisted laser desorption ionization-time of flight mass spectrometry and MetaCore pathway analysis software (GeneGo), the proteomic expression profiles of differentiated SH-SY 5Y cells after LPS treatment was studied to determine the inflammatory effects on the process of SH-SY5Y differentiation. Long-term LPS treatment resulted in the upregulation of phosphodiesterase 4B (PDE4B), slit robo GTPase (SRGAP2), transcription repressor E2F-6, vimentin, and 70 kDa heat shock protein 9 (Mortalin/HSPA9). Taken together, our results suggest that LPS-treated differentiation of SH-SY5Y cells can lend insight into the multiple pathways involved in neurological diseases.
URI
http://link.springer.com/article/10.1007%2Fs13206-012-6209-1http://hdl.handle.net/20.500.11754/67994
ISSN
1976-0280
DOI
10.1007/s13206-012-6209-1
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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