Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2018-04-16T04:18:24Z | - |
dc.date.available | 2018-04-16T04:18:24Z | - |
dc.date.issued | 2012-03 | - |
dc.identifier.citation | Cancer Research, 2012, 72(5), P.1137-1148 | en_US |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.uri | http://cancerres.aacrjournals.org/content/72/5/1137 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/67816 | - |
dc.description.abstract | Transcriptional regulation by p53 is thought to play a role in its ability to suppress tumorigenesis. However, there remain gaps in understanding about how p53 regulates transcription and how disrupting this function may promote cancer. Here we report a role in these processes for the kidney cancer-related gene KR-POK (ZBTB7C), a POZ domain and Kruppel-like zinc finger transcription factor that we found to physically interact with p53. Murine embryonic fibroblasts isolated from genetically deficient mice (Kr-pok(-/-) MEFs) exhibited a proliferative defect relative to wild-type mouse embryonic fibroblasts (MEF). The zinc finger domain of Kr-pok interacted directly with the DNA binding and oligomerization domains of p53. This interaction was essential for Kr-pok to bind the distal promoter region of the CDKN1A gene, an important p53 target gene encoding the cell-cycle regulator p21WAF1, and to inhibit p53-mediated transcriptional activation of CDKN1A. Kr-pok also interacted with the transcriptional corepressors NCoR and BCoR, acting to repress histone H3 and H4 deacetylation at the proximal promoter region of the CDKN1A gene. Importantly, Kr-pok(-/-) MEFs displayed an enhancement in CDKN1A transactivation by p53 during the DNA damage response, without any parallel changes in transcription of either the p53 or Kr-pok genes themselves. Furthermore, Kr-pok promoted cell proliferation in vitro and in vivo, and its expression was increased in more than 50% of the malignant human kidney cancer cases analyzed. Together, our findings define KR-POK as a transcriptional repressor with a pro-oncogenic role that relies upon binding to p53 and inhibition of its transactivation function. Cancer Res; 72(5); 1137-48. (C) 2012 AACR. | en_US |
dc.description.sponsorship | This work was mainly supported by the Atomic Energy Research grant (2008-2001735; to M-W. Hur), Mid-career Researcher Program grant (2009-0081294; to M-W. Hur), and Do-Yak Program grant (2011-0028817; to M-W. Hur) from the National Research Foundation of Korea (NRF) funded by the Korean government (MEST). This research was also funded by a research grant (#10016493; 2008-2009 awarded to K-R. Lee., BioCore Co., and M-W. Hur) from the Korea Ministry of Knowledge Economy. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Amer SOC Cancer Research | en_US |
dc.subject | ACUTE PROMYELOCYTIC LEUKEMIA | en_US |
dc.subject | ZINC-FINGER PROTEIN | en_US |
dc.subject | CELL-CYCLE ARREST | en_US |
dc.subject | CENTER B-CELLS | en_US |
dc.subject | HISTONE DEACETYLASE | en_US |
dc.subject | DNA-DAMAGE | en_US |
dc.subject | TUMOR-SUPPRESSOR | en_US |
dc.subject | GENE-EXPRESSION | en_US |
dc.subject | HUMAN CANCER | en_US |
dc.subject | COMPLEX | en_US |
dc.title | KR-POK Interacts with p53 and Represses Its Ability to Activate Transcription of p21WAF1/CDKN1A | en_US |
dc.title.alternative | CDKN1A | en_US |
dc.type | Article | en_US |
dc.relation.no | 5 | - |
dc.relation.volume | 72 | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-11-2433 | - |
dc.relation.page | 1137-1148 | - |
dc.relation.journal | CANCER RESEARCH | - |
dc.contributor.googleauthor | Jeon, Bu-Nam | - |
dc.contributor.googleauthor | Kim, Min-Kyeong | - |
dc.contributor.googleauthor | Choi, Won-Il | - |
dc.contributor.googleauthor | Koh, Dong-In | - |
dc.contributor.googleauthor | Hong, Sung-Yi | - |
dc.contributor.googleauthor | Kim, Kyung-Sup | - |
dc.contributor.googleauthor | Kim, Minjung | - |
dc.contributor.googleauthor | Yun, Chae-Ok | - |
dc.contributor.googleauthor | Yoon, Juyong | - |
dc.contributor.googleauthor | Choi, Kang-Yell | - |
dc.relation.code | 2012201688 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | chaeok | - |
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