Surrogate reporters for enrichment of cells with nuclease-induced mutations
- Title
- Surrogate reporters for enrichment of cells with nuclease-induced mutations
- Author
- 김형범
- Keywords
- ZINC-FINGER NUCLEASES
- Issue Date
- 2011-11
- Publisher
- Nature Publishing Group
- Citation
- Nature Methods, 2011, 8(11), P.941-943
- Abstract
- Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding fluorescent proteins can be used as surrogate genes for the efficient enrichment of endogenous gene-modified cells by flow cytometry.
- URI
- https://www.nature.com/articles/nmeth.1733http://hdl.handle.net/20.500.11754/67595
- ISSN
- 1548-7091
- DOI
- 10.1038/NMETH.1733
- Appears in Collections:
- GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > ETC
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