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Erythropoietin gene delivery using an arginine-grafted bioreducible polymer system

Title
Erythropoietin gene delivery using an arginine-grafted bioreducible polymer system
Author
이민형
Keywords
Erythropoietin; Bioreducible polymer; PEGGene therapy; Hemolysis
Issue Date
2012-02
Publisher
Elsevier Science B.V., Amsterdam
Citation
MOLECULAR THERAPY, 2012, 20(7), P.1360-1366
Abstract
Erythropoietin (EPO) plays a key regulatory role in the formation of new red blood cells (RBCs). Erythropoietin may also have a role as a therapeutic agent to counteract ischemic injury in neural, cardiac and endothelial cells. One of the limitations preventing the therapeutic application of EPO is its short half-life. The goal of this study was to develop a gene delivery system for the prolonged and controlled release of EPO. The arginine grafted bioreducible polymer (ABP) and its PEGylated version, ABP-PEG10, were utilized to study the expression efficiency and therapeutic effectiveness of this erythropoietin gene delivery system in vitro. Poly(ethylene glycol) (PEG) modification of the ABP was employed to inhibit the particle aggregation resulting from the interactions between cationic polyplexes and the negatively charged proteins typically present in serum. Both the ABP and the ABP-PEG10 carriers demonstrated efficient transfection and long-term production of EPO in a variety of cell types. The expressed EPO protein stimulated hematopoietic progenitor cells to form significant numbers of cell colonies in vitro. These data confirm that this EPO gene delivery system using a bioreducible polymeric carrier, either ABP or ABP-PEG 10, merits further testing as a potential therapeutic modality for a variety of clinically important disease states.
URI
https://www.sciencedirect.com/science/article/pii/S0168365911009862?via%3Dihubhttp://hdl.handle.net/20.500.11754/67557
ISSN
1525-0016
DOI
10.1016/j.jconrel.2011.10.014
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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